Consider a mixture comprised of the following proteins below: Protein i MW (kDa) Charge (pH 7.4) Affinity to a specific ligand %Polarity A i 760 -3 None 45 300 +1 Carbohydrate 70 80 -1 None 60 D 55 +2 None 25 a. Protein that will most strongly bind to an anion exchange column: (Choices: A, B, C, D) b. Protein that will elute last in gel filtration chromatography: (Choices: A, B, C, D) c. Protein that will elute first in hydrophobic interaction chromatography: (Choices: A, B, C, D) d. Protein that will elute last in a carbohydrate containing column: (Choices: A, B, C, D)
Consider a mixture comprised of the following proteins below: Protein i MW (kDa) Charge (pH 7.4) Affinity to a specific ligand %Polarity A i 760 -3 None 45 300 +1 Carbohydrate 70 80 -1 None 60 D 55 +2 None 25 a. Protein that will most strongly bind to an anion exchange column: (Choices: A, B, C, D) b. Protein that will elute last in gel filtration chromatography: (Choices: A, B, C, D) c. Protein that will elute first in hydrophobic interaction chromatography: (Choices: A, B, C, D) d. Protein that will elute last in a carbohydrate containing column: (Choices: A, B, C, D)
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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![Consider a mixture comprised of the following proteins below:
Protein MW (kDa) : Charge (pH 7.4)
Affinity to a specific ligand
%Polarity
A i
760
-3
None
45
B
300
+1
Carbohydrate
70
C
80
-1
None
60
D i
55
+2
None
a. Protein that will most strongly bind to an anion exchange column: (Choices: A, B, C, D)
b. Protein that will elute last in gel filtration chromatography: (Choices: A, B, C, D)
C. Protein that will elute first in hydrophobic interaction chromatography: (Choices: A, B, C, D)
d. Protein that will elute last in a carbohydrate containing column: (Choices: A, B, C, D)
25](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F3735ace9-e32a-4e6f-8ecd-7ca6d185468f%2Fb46f6d5e-3a52-4dd8-9f4c-badc8a7eb9b5%2F9cw26yv_processed.png&w=3840&q=75)
Transcribed Image Text:Consider a mixture comprised of the following proteins below:
Protein MW (kDa) : Charge (pH 7.4)
Affinity to a specific ligand
%Polarity
A i
760
-3
None
45
B
300
+1
Carbohydrate
70
C
80
-1
None
60
D i
55
+2
None
a. Protein that will most strongly bind to an anion exchange column: (Choices: A, B, C, D)
b. Protein that will elute last in gel filtration chromatography: (Choices: A, B, C, D)
C. Protein that will elute first in hydrophobic interaction chromatography: (Choices: A, B, C, D)
d. Protein that will elute last in a carbohydrate containing column: (Choices: A, B, C, D)
25
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