Can the results of an MIC test be used to determine whether an antibiotic is bactericidal or bacteriostatic? If not, set up an experimental procedure to determine whether the effect is bactericidal or bacteriostatic.
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Q: How can the use of chemical agents contribute to bacterial antibiotic resistance?
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Q: Devise an experiment that will differentiate between bactericidal andbacteriostatic effects.
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Can the results of an MIC test be used to determine whether an antibiotic is bactericidal or bacteriostatic? If not, set up an experimental procedure to determine whether the effect is bactericidal or bacteriostatic.
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- Attached below is the study about the effects of handwashing and the effectiveness of soap against bacteria. What conclusions can be generated from this result? A C before washing hands 10 second hand wash B D hot water only rinse 30 second hand wash Figure 8: Agar plates with bacteria A) before washing hands B) after rinsing hands in hot water C) After washing hands with soap for 10 seconds and D) after washing hands for 30 seconds. Images show that the number of bacteria on the plate are not greatly reduced after a hot water rinse or 10 second handwash but bacterial levels are reduced after a proper hand wash for 30 seconds.What are two possible reasons for choosing a bacteriostatic treatment over a bactericidal one? Name the factors that can compromise the effectiveness of a disinfecting agentA number of tests are used to identify a bacterial pathogen taken from human patients.Research and describe a biochemical test that is used to help determine of the genus and species of a bacteria. What bacterial types are usually identified with the use of the test you chose?
- If a bacterial species is not susceptible to an antibacterial drug at the concentration present in a particular disk, does that necessarily mean the species is completely resistant to the drug? Explain your answer. What is the mode of action of bacitracin? How does this influence the types of bacteria it can be used against effectively? What is the mode of action of tetracycline? How does this influence the types of bacteria it can be used against effectively? Describe how populations of bacteria can evolve resistance to antibiotics. In your description, use the terms/phrases: mutation, resistant, sensitive, individuals, populations, natural selection, horizontal gene transfer.Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab. What is one experiment you could do, involving culturing the organism?Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab. What is a procedure you could do, involving making a slide of the organism?
- Attached below is the study about the effects of handwashing and the effectiveness of soap against bacteria. What conclusions can be generated from this result? (see rubrics for the point marking) A before washing hands C 10 second hand wash B D hot water only rinse 30 second hand wash Figure 8: Agar plates with bacteria A) before washing hands B) after rinsing hands in hot water C) After washing hands with soap for 10 seconds and D) after washing hands for 30 seconds. Images show that the number of bacteria on the plate are not greatly reduced after a hot water rinse or 10 second handwash but bacterial levels are reduced after a proper hand wash for 30 seconds.Suppose you do the Kirby-Bauer test on a hypothetical Staphylococcus species with penicillin and tetracycline. You record diameters of 20mm for tetracycline and 24mm for penicillin. Which antibiotic is most effective against this bacterium and why? Please explain and interpret these results.What is the specific mode of action and the target organisms of each of the three antibiotics used in the exercise (isolation of E. coli bacteriophage)?a. Ampicillin:b. Polymyxin:c. Streptomycin:
- Microbiology Briefly describe any THREE CATEGORIES (three modes of action) of antibacterial drugs. Make sure to tell how they work and how bacteria have become resistant to them.Use the photo below to measure the diameter of each antibiotic (all 3) and record the name and measure the diameter of the zone of inhibition. AFTER, answer these questions: 1.What antiblotic is most effective against the bacteria? 2. What antibiotic is least effective against the bacteria? 3. What causes the zone of inhibition to form? 4. If you get no zone of inhibition what can you concludeYou inoculate a species of bacteria on a MacConkey agar. It normally shows positive for growth and ability to breakdown lactose. What other method would you use to confirm the result of the MacConkey agar, and what result would you expect?