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Briefly explain the difference between Wright Staining and H&E Staining (why use one over the other)
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- The basic components of a fluoroscopic x-ray system suitable for interventional procedures consists of a generator,x-ray tube and housing including collimator and filtration,patient table,anti-scatter grid and an image receptor.Describe the key design features and function each of these components and briefly discuss the role of automatic brightness controlThe basic component of a fluoroscopic x-ray system suitable for interventional procedures consist of a generator,x-ray tube and housing including collimator and filtration,patient table,anti-scatter grid and an image receptor.Describe the key design features and function of each of these component and briefly discuss the role of automatic brightness controlWhat are the principle and basic concepts of Simple staining? (please explain it thoroughly in a lengthy but clear explanation, thank you so much.)
- Explain the importance of steam during the acid-fast staining procedure. Discuss the advantages of differential staining procedures over the simple staining techniques. List and briefly describe the functions/purpose of each of the different stains used in acid fast staining.The significance of using immersion oil when using 1OOX objective lens is The significance of using immersion oll when uskng 100X objective lens is: It will increase the contrast by having same refractive index as the lens, loss of light by refraction, reflection and diffraction is minimized. the specimens need not be killed, fixed and stained more light is captured for better illumination, allowing the specimen to fluoresce i. il. iii. iv. A light microscope should have the following features contrast Resolution magnification fluorescence ii. iv.Which of the following is true regarding aceto-orcein staining? Question options: Is ineffective for staining as DNA is soluble in acetic acids. Orcein can be extracted from three different species of flower. Staining occurs via hydrophobic bonding and van der Waals attractions. The active dyes in this strain are small, extremely hydrophobic anionic dyes.
- What is the difference between Raman Spectroscopy and SERS ( Surface Enhanced Raman Spectroscopy).a: What are the components of a Romanowsky stain? b: What is the optimum pH for staining with Giemsa. c: Name two conditions which will affect the quality of your staining. d: What is a panoptic stain. Give one example. e: Name two blood parasites that can be demonstrated using the Giemsa stain.Explain in detail simple and differential staining and also improving and adjusting contrast in light microscopy.
- Describe how simple staining and fluorescence staining are similar and how they are different. What are the advantages of each?What are the pros and cons of using the TCE stain-free method versus a traditional gel staining method (CBB staining)?How can a UV-Vis spectrophotometer be applicable in determining the absorbance/concentration of a colorless sample? Please answer in detail and clearly. Thank you so much!