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Q: size exclusion chromatography
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Q: by
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- Use a suitable model to explain how separation and identification of a mixture of organic compounds can be achieved with a thin layer chromatographic (TLC) technique.Can paper chromatography be used to separate and identify very volatile substances? Why? Please explain comprehensively.Chemistry Briefly describe the differences between the scanning and SIM approaches with reference to your data. Discuss how and why your scanning (TIC) and SIM (mass chromatograms look different. Pay particular attention to selectivity of compounds detected and signal-to-noise. What are some advantages of SIM? What are some disadvantages?
- In normal-phase HPLC chromatography, the stationary phase is polar and the mobile phase is nonpolar. In the reversed phase we have just the opposite; the stationary phase is nonpolar and the mobile phase is polar. Describe molecules in which move faster or slower in each case.Differentiate normal phase chromatography from reverse phase chromatography. From which of the two governs the video demonstration in TLC and paper chromatography? How do you say so?Differentiate normal phase chromatography from reverse phase chromatography. From which of the two governs the thin layer chromatography of plant pigments? How do you say so?
- In molecular exclusion (size exclusion) chromatography, the molecules have the longest retention times. a) the larger molecules b) the smaller moleculesHelp me.... Explain the principles of gel permeation chromatography in Polymer, what is the application in Polymer analyticsWhat is the best Chromatography instrument for analysis of Soluble Protein molecules CG-FID GC-MS LC-MS IC-MS O IC-EC
- For what type of separation would you use ion exchange chromatography (IC)?Classify the descriptions based on whether they apply to ion‑exchange, size‑exclusion, or both types of chromatography columns. - Proteins move through the column at rates determined by their net charge. - The gel bed will contain polymer beads with charged functional groups. - Proteins move through the column at rates determined by their size. - The gel bed will contain porous polymer beads. - The properties of the proteins affect the rate at which they migrate. - Proteins are separated based on differences in their amino acid sequences.One method for quantitative determination of the concentration of constituents in a sample analyzed by gas chromatography is the area normalization method. In this method, complete elution of all of the sample constituents is necessary. The area of each peak is then measured and corrected for differences in detector response to the different eluates. This correction is accomplished by dividing the area by an empirically determined correction factor. The concentration of the analyte is found from the ratio of its corrected area to the total corrected area of all peaks. For a chromatogram containing three peaks, the relative areas were found to be 16.4, 45.2, and 30.2 in the order of increasing retention time. Calculate the percentage of each compound if the relative detector responses were 0.60, 0.78, and 0.88, respectively