1. Place 5 mL of starch solution in the test tubes. 2. Heat the test tubes to boiling and add to 1 mL of saliva, Cool and then continue heating but keep the test tube in a water bath at temperature of 40oC 3. At 5 minutes interval for 30 minutes take a drop of the reaction mixture from each test tube and test with Iodine solution (use a spot plate for the test and stir the contents of the test tube before taking a drop). Tabulate the results. 4. What would be the color of saliva extract with iodine in 3, 6, 9,12,15,and 18 minutes

1. Place 5 mL of starch solution in the test tubes. 2. Heat the test tubes to boiling and add to 1 mL of saliva, Cool and then continue heating but keep the test tube in a water bath at temperature of 40oC 3. At 5 minutes interval for 30 minutes take a drop of the reaction mixture from each test tube and test with Iodine solution (use a spot plate for the test and stir the contents of the test tube before taking a drop). Tabulate the results. 4. What would be the color of saliva extract with iodine in 3, 6, 9,12,15,and 18 minutes

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter6: Basic Clinical Chemistry

Section6.8: Fecal Occult Blood Test

Problem 1CS

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Procedure:1. Prepare 5 test tubes. Place 1 ml of 1% starch and add 10 drops of saliva toeach tube. Mix thoroughly.2. Place the first tube in ice water, the 2ndtube leave at room temperature, the 3rdtube in 40°C , the 4thtube at 60°Cwater bath and the 5thtube boil for 2minutes..3. Leave the 4 tubes in their respective temperatures for 30 minutes. The 4thtubeallows to stand for 30 minutes after heating for 2 minutes.4. Test the contents of each tube with iodine and benedict’s tests.
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Procedure: 1. Prepare 6 test tubes and place 1 ml saliva in each. 2. Add Iml water to tube 1,2 ml to tube 2, 4 ml to tube 3,6 ml to tube 4, 8 ml to tube 5 and 10 ml to tube 6. Mix thoroughly. 3. Transfer 1 ml of each into 6 separate test tubes (discard excess solution) and add 1 ml of 1% starch paste. Mix well and heat in a water bath with temperature maintained at 40°C for 30 minutes. 4. Divide each of the contents of each tube into 2 and test with: a. Iodine Test – to half of the content of each tube, add 1 drop of iodine in KI and note the color produced. Compare the intensity of color in each of the 6 tubes. b. Benedict's – to 1 ml benedict's reagent, add 5 drops of the other half of each tube and heat in a boiling water bath for 3 minutes. Note color of precipitate. 5. Rank each tube according to decreasing reaction rate one (1) being the fastest to hydrolyze and 6 being the slowest.
when testing unpasteurized milk you get a colony count of 118 colonies on plate A and 110 colonies on plate B after you plated .1 mL of a sample your .1 mL sample came from tube #6 you have prepared a serial dilution with six test tubes all tubes are a 1 to 10 dilution calculate the average CFU/ml of the original sample show work
Procedure: 1. Prepare 5 test tubes. Place 1 ml of 1% starch and add 10 drops of saliva to each tube. Mix thoroughly. 2. Place the first tube in ice water, the 2nd tube leave at room temperature, the 3rd tube in 40°C , the 4th tube at 60°Cwater bath and the 5th tube boil for 2 minutes.. 3. Leave the 4 tubes in their respective temperatures for 30 minutes. The 4th tube allows to stand for 30 minutes after heating for 2 minutes. 4. Test the contents of each tube with iodine and benedict’s tests.
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