Besides 1H NMR, FT-IR, melting point, data for refractive index, what other information could you obtain for your compound to assess its’ purity and confirm its’ structure? How would this additional data contribute to your conclusion about the structure of the unknown compound?
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Besides 1H NMR, FT-IR, melting point, data for refractive index, what other information could you obtain for your compound to assess its’ purity and confirm its’ structure? How would this additional data contribute to your conclusion about the structure of the unknown compound?
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- A student performed the fractional distillation of a cyclohexane/toluene mixture. The refractive index of the distillate was measured at 1.4301 with an ambient temperature of 22.0°C. What is the mole percentage of cyclohexane (to 3 sf)? 94.5 IncorrectBelow is a Beer's Law Plot for the concentration of a specific colored compound x. A solution of compound X is measured with the spectrophotometer to have an optical density of 0.60. a) What is the concentration of compound X in this solution. Answers must contain proper units for full credit. b) The absorbance of this compound was measured at 540 nm. Why was this absorbance chosen? 0.9 0.8 0.7 0.6 805 4 0.4 0.3 0.2 Absorbance at 540 mAssume that you have an “X” solution that you do not know its concentration. But you have other X solutions with known concentrations and you know X solution absorbs maximum light at 575 nm. To calculate the unknown solution concentration, you have done some spectrophotometric measurements and obtained data given below. Prepare a standard curve by using the absorbance of known concentrations (Hint: you can learn how to prepare a standard curve by using Excell from youtube). Calculate the concentration of unknown.
- 20 4. A sample containing ethanol and water was found to have n6 = 1.3400. Calculate the exact percentage of ethanol and water in this example. Show your work. (Hint: Solve this mathematically. Assume a linear relationship between concentration and refractive index. You can make the y-intercept the refractive index of water, and use the slope formula to find the slope. The x axis (concentration) will be from 0% ethanol to 100% ethanol. From this information, you can get the equation of the line, then plug in your numbers from there.)(b) Table Q1 below provides optical measurements of samples with known concentrations of analyte (ten samples were measured and the average and standard deviation are presented). They are also represented graphically in Figure Q1, below the table. Analyte concentration (nM) 0.1 0.2 0.5 1 2 5 10 15 fluorescence intensity 300000 250000 200000 150000 100000 50000 0 0 Table Q1 Fluorescence intensity (average) 9300 9200 10800 19600 37200 90000 178000 224000 5 [Analyte]/nM 10 Standard deviation 2000 1200 1000 2000 10000 30000 25000 40000 15 Figure Q1. Signal intensity for different analyte concentrations. Error bars are standard deviation. (ii) Calculate the limit of detection (LOD) for this sensor. (iii) A patient sample measured using this biosensor in the diagnostic laboratory generates a signal of 142800. The coefficient of variation of the device is 5%. Explain whether the diagnostic lab would have the confidence to report that the sample has a concentration in analyte of 8 nM or…A 25.0 mL aliquot was taken from a 500.0 mL sample of tonic water containing an unknown amount of quinine and diluted to a volume of 150.0 mL. At 347.5 nm, the fluorescence intensity of the diluted sample was measured as 89.3 on an arbitrary scale. Under similar conditions, a 25.0 ppm standard quinine solution had a fluorescence intensity of 173. Calculate the mass of quinine in the original tonic water sample. mass: mg * TOOLS x10
- NMR is considered a low-sensitivity spectroscopic technique. What is the physical basis of NMR's insensitivity?If I am making 500ml of a solution with 0.625ml ITS, 0.0035ml Linoleic acid, 20ml Nu-serum, 0.75g BSA, and DMEM:F12 as my solvent, how best would I make this solution and what tools would be the most accurate to use?What wavelength is most suitable for quantitative analyses for an analyte exhibiting the UV-Vis Spectrum shown below (which has an interfering analyte with the spectrum shown in a dashed line)? Explain why? 1.0r A B F 0.75- C 0.50 E 0.25 350 400 450 500 550 600 650 Wavelength, nm Figure 3-19 Choice of experimental wavelength. Solid line: measured species. Dashed line: interfering species. See text for delails. Absorbance
- Discuss the physical orig ins of the gross selection rules for infrared spectroscopy and vibrational Raman spectroscopy.Young's double slit experiment is one of the quintessential experiments in physics. The availability of low cost lasers in recent years allows us to perform the double slit experiment rather easily in class. Your professor shines a green laser (570 nm) on a double slit with a separation of 0.113 mm. The diffraction pattern shines on the classroom wall 4.0 m away. Calculate the fringe separation between the second order and central fringe. m Additional MaterialsI have: DMEM:F12 800X ITS 1.07 g/ml Linoleic acid Nu-serum (pure solution) BSA (pure powder) How much of each would I need to make 500ml of (1x ITS, 7.49 ug/ml linoleic acid, 4% v/v Nu-serum, 0.15% W/V BSA, and DMEM:F12 as the solvent)?