Aside from ovalbumin, what are other possible proteins which can be isolated from egg white? Give their biological significance.
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topic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation (Salting Out)
Aside from ovalbumin, what are other possible proteins which can be isolated from egg white? Give their biological significance.
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- Topic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation(Salting Out) The computed amount of powdered ammonium sulfate was added to the egg white sample portion byportion with constant stirring while submerged in an ice bath. The solution is expected to become moreturbid, and a white precipitate is expected to form. Explain the purpose of using powdered ammonium sulfate in the isolation of ovalbumin from egg whites.Topic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation (Salting Out) Egg whites contain about 88.5% water and about 10% proteins, from which ovalbumin is the major component. The eggs were cracked open, and the egg whites were separated carefully from the yolk. Stirring of the egg whites was done to break up the membrane. This was followed by filtration through cheesecloth, where a clear filtrate was obtained. About 40.0 mL of the filtered undiluted egg white was measured, and 0.10 mL of 1 M acetic acid was slowly added for every 1.0 mL of egg white with constant stirring. A turbid mixture with few amounts of white precipitate may be expected to form. These may be removed by filtering the mixture using a cheesecloth. What is the significance of adding 1 M acetic acid to the filtered egg white?Topic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation (Salting Out) The computed amount of powdered ammonium sulfate was added to the egg white sample portion byportion with constant stirring while submerged in an ice bath. The solution is expected to become moreturbid, and a white precipitate is expected to form. The resulting mixture was then filtered using a cheesecloth. The residue was discarded, and 30.0 mL of thefiltrate was brought from 40% to 60% saturation by adding the required amount of powdered ammoniumsulfate in the same manner as the previous addition. After adding ammonium sulfate, the mixture was allowed to stand with occasional stirring for 30 minutes inan ice bath. The formation of a white precipitate is expected to happen QUESTION: Explain the significance of allowing the mixture to be submerged in an ice bath for a given time.
- The correct order of steps when purifying a protein are: Group of answer choices 1)Growing bacterial culture for fusion protein; 2)Harvesting IPTG-induced cultures; 3)Lysing bacterial cell; 4)Removing insoluble debris; 5)Using Affinity Chromatography to purify protein. 1)Growing bacterial culture for fusion protein; 2) Breaking open bacterial cells; 3) Removing insoluble debris: 4) Harvesting IPTG-induced cultures; 5)Using Affinity Chromatography to purify protein 1) Growing bacterial culture for fusion protein; 2) Lysing bacterial cells; 3) Harvesting IPTG-induced cultures; 4) Removing insoluble debris: 5)Purifying protein by affinity chromatography 1)Growing bacterial culture for fusion protein; 2) Removing insoluble debris; 3) Harvesting IPTG-induced cultures; 4)Breaking open bacterial cells; 5)Purifying protein by affinity chromatographyTopic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation(Salting Out) Egg whites contain about 88.5% water and about 10% proteins, from which ovalbumin is the major component. The eggs were cracked open, and the egg whites were separated carefully from the yolk. Stirring of the egg whites was done to break up the membrane. This was followed by filtration through cheesecloth, where a clear filtrate was obtained. About 40.0 mL of the filtered undiluted egg white was measured, and 0.10 mL of 1 M acetic acid was slowly added for every 1.0 mL of egg white with constant stirring. A turbid mixture with few amounts of white precipitate may be expected to form. These may be removed by filtering the mixture using a cheesecloth After filtration, 30.0 mL of the filtrate was obtained, and the solution was brought from 0% to 40% saturation by adding the required amount of powdered ammonium sulfate, (NH4)2SO4 After adding ammonium sulfate, the mixture was allowed to stand…a. State the importance of using following reagents in SDS-PAGE. 1. Acrylamide 2. Bisacrylamide 3. Tetramethylethylelediamine 4. Glycerol 5. Ammonium persulfate b. Briefly describe the importance of two dimensional electrophoresis in protein separation?
- Explain the extraction method and the procedure for extraction of Echinolone by using chromatography?Discuss about the reagents used for agarose gel electrophoresis (not Polyacrylamide gel electrophoresis)Prepare the following LB media with antibiotic added. A 100ml of LB media with 25 micro/ml of Amp and 100micro/ml of Kan final concentration. A 100ml of LB is provided and amp of Kan stock at 100 mg/ml and 50 mg/ml are also provided. Find how much each antibiotic stock solution is needed to add 100ml of LB to reach desired antibiotic concentration.
- Give the advantages and disadvantages of the following dosage forms in sentence forms: ○ Tablets○ Capsules○ Syrups○ Suppositories○ EmulsionsFollowing electrophoresis of the two pGlo mini-preparation samples (both digested and undigested), the following agarose gel image was obtained. provide an accurate and detailed figure, describing the image and what it depicts.Theoretical Data Following the modified protocol for the isolation of Escherichia coli bacteriophage of Encabo (2018) presented below, compute for the pfu/ml of the chloroform-treated lysate. 1ml 1ml 1ml 1ml 1 Chloroform-treated lysate 1ml Dilution 10-3 10-4 10-5 0 0 0 0 0 9ml 9ml 9ml 10-1 10-² 10-3 Empty sterile tubes Incubate inside ref for 15-20min + 0.1 ml lysate-E.coli mix 0.1 ml 9ml 9ml 104 10.5 // 0.1 ml 0.1 ml +0.5 ml E. coli ↓↓↓↓↓↓ Incubate O/N at 35°C, observe for plaques Molten soft agar overlay B. Quantification of the Infective E. coli other Bacteriophage Particles in the Raw Sewage Sample Volume of Stock Plated (in ml) Bottom agar No. of Plaque-Forming Units (pfu) Ave. A B 254 265 132 110 11 23