As soon as lysis occurs, proteolysis, dephosphorylation and denaturation begin. These events can be slowed down considerably if samples are kept on ice or at 4°C at all times and appropriate inhibitors (protease and phosphatase) are added fresh to the lysis buffer. -Why inhibitors are necessary in this stage?-
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As soon as lysis occurs, proteolysis, dephosphorylation and denaturation begin. These events can be slowed down considerably if samples are kept on ice or at 4°C at all times and appropriate inhibitors (protease and phosphatase) are added fresh to the lysis buffer.
-Why inhibitors are necessary in this stage?-
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- Polyethylene glycol (PEG)-conjugated IFNs have superior pharmaceutical properties compared with their unconjugated counterparts as a result of greater protection against proteolytic degradation, better solubility, and slower catabolism and excretion. Group of answer choices True FalseMany proteins are secreted in their inactive form. This is also true of many toxic proteins produced by micro organisms. Explain how the mechanism is useful for the organism producing the toxin?What is the purpose of a protease inhibitor in cell lysis?
- What is the mechanism of the reaction of the Bradford reagent with proteins? (explain in not less than 5 sentences).a) What is the role of the lysosome in degrading proteins? What are the enzymes that degrade/hydrolyze proteins? How does the further degradation of amino acids occur?b) Explain the urea cycle and summarize the steps of the urea cycle starting from carbamoyl phosphate.To determine: The special properties that an enzyme isolated from a psychrophilic bacterium will have.
- hyperammonemia in patients is compounded by the reality of the normal regulatory controls in the urea cycle. Explain how increased GDH oxidative deamination activity will inhibit the urea cycle.The enzyme chymosin secreted in calf stomachs has traditionally been used to coagulate milk to make cheese. Explain how you could produce chymosin in a bioreactor to avoid sacrificing calves for the cheese making process. Be sure to describe the process in as much detail as possible and try to mention the required tools and techniques.The urea cycle requires all of the following components except one of them: aspartate, carbamoyl phosphate synthetase I, ATP, citrulline/ornithine transporter and isocitrate. Identify the component. Explain the sequence of urea cycle using the required components and enzymes essential for each step of the cycle.
- Describe in detail, the key steps of the urea сycle.Serum blood of a patient with dislipoproteinemia type 1 has milky appearance even in fasting. If serum stays at low temperature (40) for several hours fatty layer appears on its surface. What are the possible causes of these symptoms? To explain this, answer the questions and do the following tasks: a) what compounds of serum must be tested for that patient in biochemical lab? b) write the reaction which does not occur properly in patient’s blood; c) write down the schemes, explaining how the products of the previous reaction are used in adipose tissue and heart in healthy person 2 hours after a meal.Proteolysis increases during the early phases of fasting, but later it decreases as the body adapts to using alternative energy sources. Given that feedback control mechanisms have not been described for intracellular proteases, how might you explain these apparent changes in protease activity?