As soon as lysis occurs, proteolysis, dephosphorylation and denaturation begin. These events can be slowed down considerably if samples are kept on ice or at 4°C at all times and appropriate inhibitors (protease and phosphatase) are added fresh to the lysis buffer. -Why inhibitors are necessary in this stage?-

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 44SST
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As soon as lysis occurs, proteolysis, dephosphorylation and denaturation begin. These events can be slowed down considerably if samples are kept on ice or at 4°C at all times and appropriate inhibitors (protease and phosphatase) are added fresh to the lysis buffer.

-Why inhibitors are necessary in this stage?-

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