Question 1 We have received a specialized blood sera that contains antibodies. We want to test whether the antibody will bind to a target on our cell culture. The company that harvested the material has reported that the harvest works best at a 1:250 dilution. What volume of blood sera should we add to make a total volume of 1ml of antibody containing culture media? You may give your answer in any unit you like, but remember to include the unit. Edit Format Table 12pt v Paragraph v
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- QUESTION 1 The table below shows the results from looking at the diagnostic accuracy of a new rapid antigen test for COVID-19 in 100000 patients, compared to the reference standard RT-PCR test. The rows of the table represent the test result and the columns the true disease status (as confirmed by RT-PCR). COVID-19 Positive COVID-19 Negative Antigen Test Positive Antigen Test Negative 424 795 8216 90565 Calculate the SENSITIVITY of this new rapid antigen test as percentage with 3 decimal places. (Assume that the RT-PCR reference has a 100% Sensitivity and 100% Specificity.)Question 3. Biochemistry techniques have been used for the analysis on biomolecules such as enzymes, DNA, antibodies and other molecules in biomedical engineering. b) ELISA is a plate-based assay technique, designed for detecting and quantifying substances such as peptides, proteins, antibodies and hormones. Examine two principles of ELISA. c) Gel electrophoresis is a laboratory technique, used to separate the mixture of macro- molecules such as DNA, RNA and proteins according to their molecular size. i. Analyze the characteristics of DNA molecule that affect the separation results in the gel electrophoresis. ii. Examine three common factors that are possibly affect the performance of gel electrophoresis.Question 6 In the first part of the experiment, there were only a few colonies on the streptomycin positive plate. In the second part, there are a large number of colonies on the streptomycin positive plate that was inoculated with the antibiotic resistant strain. What is the most likely explanation for this difference? 1. The antibiotic resistant strain was composed of a large number of individuals with resistance to streptomycin 2. The exposure to streptomycin caused a mutation in a few individuals
- QUESTION 15 You have received an order for 6mg tobramycin, fortified to 1.5%. You have available 0.3% ophthalmic solution and 80mg/2mL injection solution. How many mL of each do you need to make the fortified solution? Save AnswerQUESTION 3 You are provided with plasmid DNA at 200ng/uL, 10X restriction digestion buffer, restriction enzymes (1 unit/ul) and water. You want to digest 1.5 ug of plasmid DNA with 1 unit (U) of enzyme in 1X buffer in a total volume of 80 ul. Complete the table below: Solution Stock solution Working solution Volume needed restriction digestion buffer 10X 1X DNA 200 ng/ul 1.5 με enzymes 1 unit/ul 1 unit Water Total 80 μLQUESTION 2 The SARS-COV-2 pandemic resulted in the desire to design ELISA assays to either detect antibodies against SARS-CoV-2 protein (previous infection), or detect viral protein (current infection). You plan to express the mutant spike protein variant using the plasmid pictured below as your template. Here's the sequence you want to put in its place: https://www.ncbi.nlm.nih.gov/nuccore/AY429073.1?report=fasta, this is the DNA sequence you're going to buy as a gene block! But you need to add a couple things to it first so it'll get into the plasmid. To do so, you're going to need to remove the current insert (represented by the yellow arrow beginning at position 833-4867). Question: What restriction sites are you going to add at the ends of the gene? Answer can be in nucleotides or the enzyme name. For the first blank fill in the site name/sequence you'd add at the beginning of the gene (5' / C-terminus end) and for the second blank fill in the sequence/site name you'd add at the…
- Question 1 A 60-year-old woman was recently given a diagnosis of advanced non- small cell lung cancer. She is going to begin treatment with cisplatin 100 mg/m 2 plus vinorelbine 30 mg/m2. Discuss your pharmacological planQuestion 13 Maria used the ABO blood testing kit to determine her blood type. Her test showed the following anti-A anti-B anti-D + means agglutination was observed - means no agglutination was observed What is Maria's blood type? AB+ O - O A+ AB-Question 12 (1 point) Listen You are working in the lab with Streptococcus pyogenes and two other bacteria. You streak all three of these microbes onto a Blood Agar plate and observe the following: All of the bacteria grow on the plate but there is a large zone of clearing around S. pyogenes, and no zone of clearing on the other two bacteria. Based on these results what type of Medium is Blood Agar? O A) Blood agar is a culture medium. B) Blood Agar is a selective medium. C) Blood agar is a differential medium. O D) Blood agar is an exclusion medium. O E) Blood agar is a counting medium. 98% 99+
- Question 15 Juan used the ABO blood testing kit to determine his blood type. His test showed the following anti-A anti-B anti-D + means agglutination was observed - means no agglutination was observed What is Juan's blood type? O A- A+QUESTION 8 Check all the statements that are TRUE regarding 16S or ITS (microbiome) illumina (NGS) sequencing vs. whole genome illumina (NGS) sequencing. O A. You don't need to trim the reads for 16S sequencing, but you do for whole genome sequencing. O B. Whole genome sequencing files will have more reads in them because genomes are bigger than 165 amplicons. O C. The read alignment and contig assembly steps would probably be harder for whole genome sequencing. O D. The 165 molecules being sequenced are always DNA, while for whole genome sequencing they could be RNA or DNA going into the illumina sequencer. O E. NGS for 16S and whole genome sequencing can both use sample indexes/barcodes to maximize efficiency. O F. Whole genome sequencing doesn't necessarily require you to know any of the sequences/targets before hand to design specific primers for.QUESTION 7 Plotted in this graph are data from two Fluorescence Recovery After Photobleaching (FRAP) experiments. Which of the following explains the difference between the two sets of data? fluorescence O time The cell indicated by the solid line was assayed at a lower temperature than the cell indicated by the dashed line. The cell indicated by the solid line has more saturated fatty acids in its phospholipids. The cell indicated by the solid line has longer fatty acids chains in its phospholipids than the cell indicated by the dashed line. The membrane of the cell indicated by the solid line is comprised of fatty acids rather than Ophospholipids.