After an antimicrobial drugs test, the following results were obtained from a disk-diffusion test against a bacterium. Antiboitic A= 6mm Antibiotic B= 18mm Antibiotic C= 11mm Antibiotic D= 19mm which drug should be used to treat an infect caused by this bacterium (assuming everything else is equal about these antibiotics)?
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After an antimicrobial drugs test, the following results were obtained from a disk-diffusion test against a bacterium.
Antiboitic A= 6mm
Antibiotic B= 18mm
Antibiotic C= 11mm
Antibiotic D= 19mm
which drug should be used to treat an infect caused by this bacterium (assuming everything else is equal about these antibiotics)?
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- What are two possible reasons for choosing a bacteriostatic treatment over a bactericidal one? Name the factors that can compromise the effectiveness of a disinfecting agentdiameter AM=47MM TE= 27 MM both left hand side is ampicillin and right hand side is tetracycline With reference to the table below, determine the antibiotic susceptibilities of different antibiotics against coli and S.aureus. S.aureus Antimicrobial agent Interpretive categories and zone diameter breakpoints, nearest whole mm sensitive Intermediate resistant Tetracycline >19 15-18 <14Which one of the following methods of antimicrobial susceptibility testing requires a dilution series of the antibiotic and can provide a minimum inhibitory concentration? Select one: a. Disk diffusion method b. Broth microdilution method c. Agar macrodilution method d. Gradient diffusion method
- A class of 15 students (8 males and 7 females) will need to culture an unknown bacteria for a specific activity where 5 nutrient agar pates per female student and 3 agar slants per males student are needed to prepare. agarplate: 25mL Agar slant: 10mL Broth tube: 8mL Nutrient Agar: Yeast extract: 2g/L peptone: 5g/L Sodium chloride: 5g/L Agar powder: 15g/LWhich one of the following methods of antimicrobial susceptibility testing combines some of the properties of two other methods and involves adding a plastic strip to the agar? Select one: a. Agar macrodilution method b. Gradient diffusion method c. Broth microdilution method d. Disk diffusion methodA researcher is planning to plant antibiotic-resistant Escherichia coli pure bacteria sample in antibiotic (ampicillin) solid media in his experiment. Describe each of the sterilization methods that this researcher should use while preparing this solid medium with antibiotics and in cultivation of the bacteria on the solid medium, indicating the stages at which it should be used and giving the mechanism of action.
- both left hand side is tetracycline and right hand side is ampicillin With reference to the table below, determine the antibiotic susceptibilities of different antibiotics against coli and S.aureus. Am=17mm Te=23mm E.coli Antimicrobial agent sensitive Intermediate resistant Interpretive categories and zone diameter breakpoints, nearest whole mm Ampicillin >17 14-16 <13 Tetracycline >15 12-14 <11Ex. 17: Chemotherapeutic Agents Using the Antimicrobial Susceptibility Table on pg 62 in the lab manual, determine whether not the bacteria plated in this Kirby-Bauer test are resistant (R), intermediate (I), or susceptible (S). The bacterial species tested was Escherichia coli (Gram negative). Ciprofloxacin Diameter = 20 Streptomycin Diameter 30 Carbenicillin Diameter 17 + Erythromycin Diameter = 23 Novobiocin Diameter = 0 bacterial growth = disk with antibiotic (see labels) = no growthA culture of Staphylococcus of unknown density is diluted as follows (letters represent each step): • 60 mL of a bacterial culture is diluted by adding 440 mL of water • 1000 microliters from (A) is added to 9 mL of water • Dilution (B) is used to prepare a 103 dilution in water • 100 ul from dilution (C) is plated to nutrient agar and incubated at 37°C Following incubation, colonies are uniformly distributed on the agar, and 102 colonies are counted on one half of the plate. 1. Express the total CFU/mL of the original culture in scientific notation. 2. If 10 ul is plated from dilution (C), how many colonies should grow on the plate? Edit View Insert Format Tools Table
- (c) Public health scientists wanted to investigate the effect of ionising radiation on the growth of Salmonella typhimurium in the presence of antibiotics. They grew bacterial colonies of Salmonella typhimurium in petri dishes labelled A, B and C. The Petri dishes were kept at 36°C for 48 hours, with scientists observing the bacteria every 24 hours. The results for this investigation are below. Petri dish A B с Treatment Was subjected to ionising radiation followed immediately by a dose of antibiotic X An equal amount of antibiotic X was added (as was added to Petri dish A) Was given neither ionising radiation nor antibiotic X After 24 hours Small spots of growth on surface No growth Growth across whole surface After 48 hours (ii) Identify the purpose of petri dish C. Large spots of growth on surface No growth Growth across whole surface (i) Explain the effect of the ionising radiation on the bacteria. (iii) Explain why scientists must continue to develop new antibiotics.There are so many microbes in a single mL of culture, it is very difficult to perform one dilution to produce countable cells. Microbiologists need to perform a dilution series, where multiple dilutions are performed in sequence to arrive at the correct dilution. Dilutions are cumulative. Multiple the series of dilutions together to find the final dilution value. If 3 serial dilutions are performed, each with a value of 0.01, what is the cumulative dilution? Express your answer as an exponent, e.g. 0.1 would be 1e-1 and 0.01 would be 1e-2Why is Muller Hinton Agar (MHA) ideal for antibiotic susceptibility testing