Absorbance spectrum of Ninhydrin curve 1,6 1,4 1,2 0,8 0,6 0,4 0,2 100 200 300 400 500 600 700 800 900 0,2 Wavelength nm Arginine Leucine Proline 2. Discuss the absorption spectra of the 3 amino acids as given in graph 1. Absorbance (OD)
Q: Which is true about nitrogenase? I. Holds N2 to a metal ion during catalysis II. Has N2 as its only…
A: Nitrogenase class of enzymes reduces Nitrogen (N2) to ammonia (NH3) produced by bacteria.
Q: Determine the cause-effect relationship of the following variables given. Choose the best answer…
A: Gout is a Metabolic disorder that is associated with increased serum concentration of uric acid that…
Q: 17. Cleavage of fructose-1,6-biophosphate yields ____. Select one: a. only a ketose b. two aldoses…
A: Glycolysis occurs in both aerobic as well as anaerobic respiration. Glycolysis is the only…
Q: The following speaks true of metabolism except. 1. Property consistent to all organisms ranging…
A: Metabolism involves the processes of both anabolism and catabolism.
Q: Define protein and their role?
A: A biomolecule, sometimes known as a biological molecule, is any of the many compounds created by…
Q: Why is potential Hydrogen (pH) important to Biology?
A: The pH of aqueous or other liquid solutions indicates how acidic or basic they are. The phrase,…
Q: What are vitamins?
A: Vitamins are needed for the normal cell function, growth, and development.
Q: 9. Catabolism, the degradative phase of metabolism, is said to be convergent. This mea that: A. the…
A: Introduction: The term catabolism refers to the breakdown of large complex molecules to form…
Q: Name the carbohydrate and identify its classification (monosaccharides, disaccharides, or…
A: Carbohydrates are composed of carbon, hydrogen, and oxygen in the ratio of 1:2:1. Carbohydrates act…
Q: When the acetyl-CoA produced during β-oxidation in the liver exceeds the capacity of the citric acid…
A: Starting at the carboxyl end, two carbons from acyl-CoA molecules are cleaved at a time in the…
Q: exactly is meant by the term "homogeneous structure"
A: Homogenous in biological term refers to as common or similar.
Q: For each genotype below, indicate whether it is heterozygous or homozygous. Write PAUL if its…
A: A set of nucleotides in the DNA that encodes a product (RNA or protein) is referred to as a gene…
Q: A solution contains a mixture of four proteins: urease, ovalbumin, lysozyme, and alkaline…
A: Proteins are composed of a linear chain of amino acids attached together via peptide bonds. There…
Q: Describe the functions of the following hormones: Aldosterone Erythropoietin Antidiuretic hormone…
A: Hormones are chemical messengers that are released directly into the bloodstream, where they are…
Q: Why is it that the secondary messenger system is named as such? What is the two most common…
A: Cell signalling pathways help in communication between the cells and the surrounding environment.…
Q: ou know that the free energy of ATP hydrolysis depends on the ATP/ADP ratio. Given that under…
A: ATP is the energy currency of the cell.
Q: Complété thế table Biosynthesis Sources Functions Deficiency oxicity
A: Introduction: Vitamins are important organic nutrients required in minimal amounts for normal…
Q: There is energy requirement for every amino acid added in a growing polypeptide chain. Is this true…
A: Introduction: The information transferred from DNA to messenger RNA (mRNA) is translated into an…
Q: ow many grams of a 1:3 mixture of glucose/glycerine would be required to make 2000mL of water…
A: Glucose is an essential component of the body and is known as blood sugar. Glycerin is used as a…
Q: 1.A student has fasted for a day to lose weight. How did the carbohydrate mctabolism change when the…
A: After a meal, absorptive stage lasts for about 4 hours. In absorptive stage catabolism beats…
Q: Which of the following is true? Group of answer choices All prokaryotes have a common genetic code,…
A: The amino acid sequence of proteins is determined by the sequence of nucleotides in deoxyribonucleic…
Q: fill in the blanks The pyruvate dehydrogenase complex is primarily regulated at the (…
A: PDC is a multiple enzyme complex that catalyzes the production of acetyl-CoA from pyruvate produced…
Q: Which of the following contains isoprene units?
A: The terpenes are monoterpenes are compounds that contain two isoprene molecules.
Q: Suggest the possible class of enzyme (or name of enzyme) for each of the enzyme-catalyzed reactions…
A: Introduction: Enzymes are biological catalysts that fasten the rate of the biochemical reaction.…
Q: A series of bovine serum albumin (BSA) was prepared and 1 mL of each solution was subjected to a…
A: The Bradford assay is based on the fact that the absorbance maximum for an acidic solution of…
Q: ACTIVITY 9.4 Complete the table Vitamin Coenzyme it forms (or helps)
A: Some enzymes require the participation of coenzymes for their activity.
Q: Briefly discuss how SLBs may be used to study drug-membrane interactions.
A: Lipophobic / Hydrophilic drugs cannot pass the Lipophilic/hydrophobic phospholipid bilayer of cells.…
Q: Protein synthesis in bacterial cells usually starts with a" A. Alanine Residue B. Formylmethionine…
A: Protein synthesis is a 5 step process (Activation of amino acids, Transfer of amino acids to tRNA,…
Q: The enzyme lactate dehydrogenase (LDH) is produced as an isozyme, which is a way to control the…
A: Lactate dehydrogenase (LDH) is an enzyme of the anaerobic metabolic pathway and is an oxidoreductase…
Q: TRUE or FALSE: "The initiation codon for both prokaryotes and eukaryotes is AUG coding for the same…
A: Introduction: The genetic code consists of 64 triplets o nucleotides. These triplets are referred to…
Q: Draw the Fischer projection of glucose and show its catabolism until it releases carbon dioxide via…
A: In order to track where every carbon of glucose went we use isotropic tracers experiments. Carbon of…
Q: TRUE OR FALSE = "The initiation codon for both prokaryotes and eukaryotes is AUG coding for the same…
A: The triplet codon AUG always codes for methionine which is also called the start codon for…
Q: The following nutrient molecule is digested and transported to a cell where it undergoes further…
A: The molecule given in the question CH3-(CH2)14-COOH is hexadecanoic acid, which is commonly known as…
Q: ACTIVITY 10.2 Refer to Figures 10.2.1 and 10.2.2 of Model 10.2 and answer the question that follows…
A: Anabolic processes are reactions in which large macromolecules are synthesized from smaller…
Q: 31. AN ENZYME WHICH IS A LOW MOLECULAR WEIGHT AND DIALYZABLE? A. APOENZYME B. HOLOENZYME C.…
A: “Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: Calculate the GI50 value from the graph showing different concentrations of N3P. Show full working.…
A: The concentration of an inhibitor causing 50% cell growth inhibition is called GI50 of the…
Q: Enzyme X has a Km of 2 µM and a kcat of 104 s1. In the presence of an inhibitor, Km increases to 4…
A: Enzymes are protein molecules that speed up the reactions by decreasing the activation energy.…
Q: fill in the blanks Anaerobic metabolism of pyruvate in muscles form ( ) while…
A: Pyruvate is also known by the name pyruvic acid. In biochemistry it is considered as a very…
Q: 10. Which reaction is convergent? Why?
A: Metabolism are consist of biochemical reactions which involved both catabolism (breakdown) and…
Q: Te ing Comme deptn pndat AiyiCoA Ceel c gae thpral nd Co Figure 10.2.2 Stages of Catabolism (Taken…
A: Kreb cycle/ citric acid cycle / tricarboxylic acid cycle is the common pathway of degraded complex…
Q: What are the functions of the following steps in the purification and isolation of proteins:…
A: The lysis of cells is the first step in the purification of a cellular protein.
Q: What is the nature of the primers used in RT-PCR tests? (i.e. are they DNA primers or RNA primers?)
A: In RT-PCR, the RNA sample obtained from a virus is converted into DNA, and the DNA is amplified and…
Q: CH;OH H ÓH H H OH ČH,OH H ÓH
A: Polysaccharides are made of monosaccharide units linked together by glycosidic linkages.
Q: Answer brieftly How do B vitamins make energy production possible?
A: Vitamins are micronutrients that are required in small amounts by the body. Vitamins are classified…
Q: QUESTION 28 All of the following statements about the Illumina (reversible terminator) process for…
A: Next-Generation sequencing also called high throughput sequencing or second-generation sequencing or…
Q: Which statement below is false about the probability of the absorption of a weak base from the…
A: Acid is a compound that donates proton and base is a compound that accepts proton. The strength of…
Q: Statement I: Glucose, galactose & fructose are absorbed in the stomach Statement II: Galactose and…
A: Metabolism is the set of chemical reactions in organisms that includes anabolism and catabolism.
Q: 1. Considering the following nucleotide sequence in an mRNA molecule: 5’ AUG UUA CGU AAU GCU GUC…
A: The genetic code is a set of three-letter combinations of nucleotides that corresponds to a specific…
Q: OF THE FOLLOWING NITROGEN BASES CAN ONLY BE FOUND IN AN DNA MOLECULE? A. ADENINE B. URACIL C.…
A: DNA is a double stranded helix made up of polymer of nucleotides consisting purines and pyrimidine.
Q: Explain your answer briefly but concisely 2. What are the steps in the energy-yielding phase?
A: The energy yielding phase of glycolysis consists of five steps and gives four ATP, two NADH + H+,…
Step by step
Solved in 2 steps with 3 images
- Quantitative Estimation of Amino Acids by Ninhydrin http://vlab.amrita.edu/?sub=3&brch=63&sim=156&cnt=2 can u help me with question 2 of the assignment questions Based on the experimental data provided, estimate the amount of amino acid in the given unknown solution by Ninhydrin method. SI No. Volume of standard amino acid solution (ml) Amount of amino acid (µg) OD at 570nm 1 Blank 0 2 0.2 0.12 3 0.4 0.25 4 0.6 0.45 5 0.8 0.55 6 1.0 100 0.68 7 Unknown (0.5ml) 0.41discuss the absorption spectra of the 3 amino acids Arginine, Leucine and Proline.On Dimyristoyl phosphatidylserine, Palmytoyl-Oleoyl Phosphatidylcholine and Sphingomyelin, What is the capacity of each of these to form hydrogen bonds with its neighbours
- 80mL of a 0.3M solution of hexapeptide Leu-His-Cys-Glu-Asn-Arg is adjusted to pH=pl. The solution is then titrated with 0.2M HCI to a final pH of 2.1. Sketch the titration curve, labelling the pH and volume axes. Indicate the volume of HCl needed to reach each relevant pKa value and equivalence point(s). Relevant pka values are: 2.1, 4.3, 6.0, 8.3, 9.8, and 12.5.Correlation between lipid profile and Covid-19.Explain this statement.TABLE 3-1 Properties and Conventions Associated with the Amino Acids pk, values Amino acid Phenylalanine Tyrosine Tryptophan 5.66 5.48 9.59 5.89 Abbreviation/ symbol 6.14 Phe F Tyr Y Trp W pk, M, (-COOH) 165 181 204 . 1.83 2.20 2.38 Using the table 3-1, calculate the pl of Tyrosine. Hint: Consider the structure and overall charge of the molecule between each pka. pK₂2 (-NH) 9.13 9.11 9.39 PKR (R group) 10.07
- Titration curve of an unknown amino acid The graph below shows a curve, which was obtained following titration of an unknown amino acid. Include a suitable descriptive title stating the identity of the unknown amino acid Use rectangles to precisely outline the regions in the graph where ionisable groups show buffering activity (base/acid ratio 1:10 to base/acid ratio 10:1); clearly associate the name of the ionisable group with the buffering regions; indicate within the graph all observable titration mid-points and all observable titration end-points and indicate estimates of the pKa of the three functional groups (do not provide pka values from the literature, you need to read the pka from the titration curve provided). Note that it is not possible to estimate the pka with more than one decimal precision due to limited resolution of the shown graph.Structures of L-Cysteine from highly protonated to depronated form. Note Structure A as themost protonated form. Based on the table, 1. Show charges and pKa summary then calculate for pI value.what is lactose intolerance ? describe the molecular life cycle for this disease. also describe how it occurs in a molecular level detailed mechanism. what causes this disease and how it develops ? provide detailed biochemical phenomena and life cycle for Lactose Intolerance condition.
- Predicted observation of precipitation of albumin with: - picric acid -tannic acid - 70% ethyl alcoholSaponify this mixed triglyceride with 3NaOHOne round of Edman degradation of the peptide: H2N- Gly-Arg-Lys- Phe-Asp- COOH which of the following structures would result when add cyanogen bromide ? A) H2N-Gly-Arg-COOH + H2N–Lys-Phe- Asp-COOH B) H2N-Arg-Lys-Phe-Asp-C0OH+ Gly C) H2N- Gly-Arg-Lys-Phe-Asp- COOH D) H2N-Gly-Arg-Lys-Phe-COOH + Asp E) H2N-Gly-Arg-Lys-COOH + H2N-Phe-Asp-COOH