A microorganism was cultured in a flask containing a liquid medium. Samples weretaken hourly and number of cells counted using a counting chamber. The data areprovided below in cells per mL.Draw a growth curve in Excel and use it to calculate generation time. The answer mustbe provided as a number (in minutes).Time Cells per mL8123584906710261112131415161718192021222324252.00E+042.20E+042.30E+042.40E+043.10E+045.00E+048.06E+041.30E+052.09E+053.38E+055.44E+058.78E+051.41E+062.28E+063.68E+065.93E+069.56E+061.54E+072.48E+074.01E+076.46E+071.04E+081.68E+082.71E+082.72E+082.73E+08 Note: 3.00e+06 is equivalent to 3.00 x 106

A growth curve is a diagram that shows how an organism population grows over time. It displays the…

A microorganism was cultured in a flask containing a liquid medium. Samples were taken hourly and number of cells counted using a counting chamber. The data are provided below in cells per mL. Draw a growth curve in Excel and use it to calculate generation time. The answer must be provided as a number (in minutes). Time Cells per mL 0 ~~~~~~ W RE E 。 ∞ Va A WNI 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 2.00E+04 2.20E+04 2.30E+04 2.40E+04 3.10E+04 5.00E+04 8.06E+04 1.30E+05 2.09E+05 3.38E+05 5.44E+05 8.78E+05 1.41E+06 2.28E+06 3.68E+06 5.93E+06 9.56E+06 1.54E+07 2.48E+07 4.01E+07 6.46E+07 1.04E+08 1.68E+08 2.71E+08 2.72E+08 2.73E+08

A microorganism was cultured in a flask containing a liquid medium. Samples were taken hourly and number of cells counted using a counting chamber. The data are provided below in cells per mL. Draw a growth curve in Excel and use it to calculate generation time. The answer must be provided as a number (in minutes). Time Cells per mL 0 ~~~~~~ W RE E 。 ∞ Va A WNI 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 2.00E+04 2.20E+04 2.30E+04 2.40E+04 3.10E+04 5.00E+04 8.06E+04 1.30E+05 2.09E+05 3.38E+05 5.44E+05 8.78E+05 1.41E+06 2.28E+06 3.68E+06 5.93E+06 9.56E+06 1.54E+07 2.48E+07 4.01E+07 6.46E+07 1.04E+08 1.68E+08 2.71E+08 2.72E+08 2.73E+08

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter18: Instrumentation In The Operating Room

Section: Chapter Questions

Problem 3CS

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You are given a bacterial culture which has a concentration of approximately 5.0 x 10^8 cells/mL. List a series of dilutions and platings that you could carry out in order to determine the exact concentration of the culture. Note that you must plate four plates from a minimum of two dilution tubes. The volumes plated should be in the range of 0.1 mL – 1.0 mL. Duplicate volumes may not be plated from any one dilution tube. Each plating should aim for a count between 30 and 300 CFUs. You can select any value from 30-300 for CFU and any volume from 0.1-1.0 to find out dilution scheme
As shown in this diagram, you perform a ten-fold serial dilution of a culture to determine the number of colony forming units (CFU) per mL it contains. You do a plate count with these growth results (no. of colonies for each dilution): 1:10 too many to count; 1:100 too many to count; 1:1,000 312; 1:10,000 38; 1:100,000 no growth. The number of CFU per mL in the original culture was: A. 38 B. 380,000 C. 38,000 D. None of the other four answers (Correct answer not given) E. 10,000
As shown in this diagram, you perform a ten-fold serial dilution of a culture to determine the number of colony forming units (CFU) per mL it contains. You do a plate count with these growth results (no. of colonies for each dilution): 1:10 too many to count; 1:100 too many to count; 1:1,000 174; 1:10,000 23; 1:100,000 no growth. The number of CFU per mL in the original culture was: 1 ml 1 ml Original inoculum Dilutions 9 ml broth in each tube 1:10 1 ml 174,000 1:100 1 ml 1 ml 1 ml 1:1000 1 ml 1:10,000 1 ml None of the other four answers (Correct answer not given) 1,000 230,000 174 1 ml 1:100,000 1 ml
The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 1 mL aliquot from each dilution was mixed with warm molten agar and poured into a Petri dish. The numbers of bacterial colony forming units (CFU) after overnight incubation are shown in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate Dilution Plate 1 10 dilution Plate 2 10 dilution -5 Plate 3 10 dilution Plate 4 10 dilution Plate 5 107 dilution -8 Plate 6 100 dilution *Too many to count Number of colony forming units (CFU) TMTC* TMTC* TMTC* 867 154 18
The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 1 mL aliquot from each dilution was mixed with warm molten agar and poured into a Petri dish. The numbers of bacterial colony forming units (CFU) after overnight incubation are shown in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate Dilution -3 Plate 1 10 dilution Plate 2 10-4 dilution Plate 3 10-5 dilution Plate 4 10 dilution Plate 5 10 dilution -8 Plate 6 100 dilution *Too many to count Number of colony forming units (CFU) TMTC* 1,957 65 21 1 0 m
Determine the CFU/ml of the original culture in the image below. Write your value in scientific notation using the "^" symbol to denote an exponent. (Example: 1x10^3). Write the number only (no labels, like CFU/ml). 100 μ. . A Original Culture 1.2x10^6 100 μ. 100 μ. Lawn of growth 100 μ. B΄900 ul c|900 μl | D |900 μ. € 900 μ. | 100 με 1100 με 1100 με 1100 μ. 1,198 120 colonies colonies O 00 10 colonies
The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 0.1 mL aliquot from each dilution was spread onto Plate Count Agar (PCA). The number of bacterial colony forming units (CFU) after overnight incubation are shown as listed in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate 1 10 Plate 2 10 Plate 3 10 dilution dilution dilution Plate 4 10 dilution Plate 5 107 dilution Plate 6 10 dilution -6 *Too many to count Number of colony forming units (CFU) TMTC* TMTC* 840 28 19 1
The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 0.1 mL aliquot from each dilution was spread onto Plate Count Agar (PCA). The number of bacterial colony forming units (CFU) after overnight incubation are shown as listed in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate Dilution Plate 1 10 dilution Plate 2 10 dilution Plate 3 107 dilution Plate 4 10 dilution Plate 5 107 dilution Plate 6 100 dilution *Too many to count Number of colony forming units (CFU) TMTC* 382 83 10 2 0
This is the growth curve for Clostirdium (incubated under optimal growth conditions). Use it to answer the following questions. 3 Cell concentration (million cells/ml) 2.5 2 0.2 1.5 1 0.5 0 2 The growth rate is lag log 0.25 4 0.32 0.5 units 6 million of cells per ml (Image description.: Horizontal axis is time in hours, Vertical is cell concentration in millions of cells per ml. Curve starts at 0,0, increases by 4 hours to 0.5 million cells/ml, goes up steeply over two hours from 0.5 million/ml at 4 hours to 2.5 million cells/ml at 8 hours, 8-12 hour shows an increase of 0.3 to 2,8 million cellls/ml and last 2 hours is flat at 2.8million cells/ml.) From 12 hr to 14 hr is called phase. lunar 2 8 Time (hours) 10 5 stationary 12 cells 14 death 16 million of cells per ml per hour hours million of cells
Using the attached image, explain the observed results on the NA and ECM plates. Be sure to address why each sample is either able or unable to grow on each plate and include the genotype(s) of the cells which are capable of growth.
When viable cell concentrations are too high to count on an agar plate, it is common to use larger sized plates to increase the surface area for counting the colonies.True or false
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