A friend of yours who is taking a biochemistry class saysthat salting out works because the waters of hydration thatsurround the protein prefer to surround the electrolyte asthe electrolyte is added; therefore, the protein’s hydrationshell is stripped away, leading to protein precipitation. Anotherfriend of yours in the same biochemistry class saysthat salting out works because the incoming ions adsorbtightly to the protein, making ion pairs on the protein surface,which end up giving the protein a zero net charge inwater and therefore leading to precipitation. Discuss thesetwo hypotheses. What kind of measurements would youneed to make to distinguish between these two hypotheses?
A friend of yours who is taking a biochemistry class saysthat salting out works because the waters of hydration thatsurround the protein prefer to surround the electrolyte asthe electrolyte is added; therefore, the protein’s hydrationshell is stripped away, leading to protein precipitation. Anotherfriend of yours in the same biochemistry class saysthat salting out works because the incoming ions adsorbtightly to the protein, making ion pairs on the protein surface,which end up giving the protein a zero net charge inwater and therefore leading to precipitation. Discuss thesetwo hypotheses. What kind of measurements would youneed to make to distinguish between these two hypotheses?
Chemistry: The Molecular Science
5th Edition
ISBN:9781285199047
Author:John W. Moore, Conrad L. Stanitski
Publisher:John W. Moore, Conrad L. Stanitski
Chapter10: Fuels, Organic Chemicals, And Polymers
Section: Chapter Questions
Problem 119QRT
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A friend of yours who is taking a biochemistry class says
that salting out works because the waters of hydration that
surround the protein prefer to surround the electrolyte as
the electrolyte is added; therefore, the protein’s hydration
shell is stripped away, leading to protein precipitation. Another
friend of yours in the same biochemistry class says
that salting out works because the incoming ions adsorb
tightly to the protein, making ion pairs on the protein surface,
which end up giving the protein a zero net charge in
water and therefore leading to precipitation. Discuss these
two hypotheses. What kind of measurements would you
need to make to distinguish between these two hypotheses?
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