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- The erythrocyte sedimentation rate (heaviness) test of a blood sample is often used as a diagnosis of immune disorders and some other diseases. Assuming that the red blood cells are about a sphere with a diameter of (approximately 5 um) and a density (g/ml 1.125), and the density and viscosity of the plasma in which the red blood cells are settled (Pa.s), (1025 kg/m3 10-3 x 1.5) On arrangement and acceleration m/s2 9.81 Calculate the erythrocyte sedimentation rate (settlement rate).You are given a 27 % (w/v) solution of psicose. Molar mass: psicose (C6H12O6) = 180.156 g/mol. ou have a 0.75 mg/mL solution of bovine serum albumin (BSA). You add 1 mL of the 0.75 mg/mL BSA solution into a standard cuvette (1 cm path length), set your spectrophotometer to 280 nm and read the absorbance of this BSA solution. Molecular mass of bovine serum albumin = 66.4 kDa. Molar extinction coefficient (e) of BSA = 43824 M-1 cm-1 (at 280 nm). What absorbance reading will you obtain?Propose a serial dilution that would give total dilution of 1/158000000 (six zeros) Each tube in your dilution series must have a volume of 50 ml or less.
- Use the following information collected from a SIZE-EXCLUSION CHROMATOGRAPHY experiment to answer the questions below. MW Standards Molecular Weight (kDa) Elution Volume (mL) blue dextran 1000 85 myoglobin 17.2 200 TIM 53.3 178 hemoglobin 62.2 156 IgG 140 122 ATCase 307.9 94 Based on the notes provided to you, which Sephadex gel filtration media would be ideal to use with the given standards? and whyYour patient Jane Doe weighs 198lb. You have 1 L bag of saline (from which you have removed 10ml) and a drug pump which can be set to run between 1 and 10 ml/min. She has an order for the drug CONVERTIN to be given at a dose of 300 ug kg hr. A vial of CONVERTIN contains 90mg of the drug in 10ml of saline. After adding the vial of CONVERTIN to the bag of saline and connecting it via pump to the patient's IV line, at what rate should the pump be set in order to deliver the drug dose as ordered?Using this dehydration method, any error less than 10% is acceptable. A. If your percent error is less than 10% discuss what portions of the experiment were most important to achieve accurate results My percent error was 3.32% so answer the question above
- After three minutes, the concentration of drug Zip in the red blood cells is 10 mmoles l-1. What is the average rate of entry of drug Zip into the red blood cells in units of moles min-1 red blood cell-1 during the first three minutes after placing the red blood cells into the bathing solution? Assume that each red blood cell occupies about 1 x 10-13 liters.Show the formula and steps that you will use to complete these calculations. The physician has prescribed a dose of 100 mg of intramuscular morphine. The available stock has a strength of 10 mg/mL. How much of this solution should you draw up into the syringe? The prescribed dose of a drug is 120 mcg. The available stock has a strength of 20 mcg/mL. How much should you give the patient? Substitute the known quantities in the formula and perform a calculation to determine the volume. Show your work).Question 1: Suppose you have given 5 tablets weighing 449mg, 450mg, 448mg, 451mg and 453mg, respectively. Calculate 300 mg equivalent sample weight for assay. The product claim is 500 mg Vitamin C is present in each tablet. Question 2: Volume of the standard sodium edetate is 12.6 mL (calculated after the assay of Zinc Sulfate Syrup). Determine the % of potency of the drug considering the claimed dose is 11.5
- For a gradient system with a gradient of 5-90% in 50 min, flow 2 mL/min, column 100 x 4.6 mm i.d., the first peak elutes at 20 min, and the last peak elutes at 50 min. Calculate k* for this system. 2. Can isocratic elution be used for this sample? If so, what is the range of k values to be expected. 3.Propose a way to shorten the gradient run time by eliminating the wait for the first peak to elute at 20 min. 4. For the original conditions of this question, change the conditions necessary to use a 150 x 4.6 mm column while maintaining the same k* value.Beans the 21kg Pit Bull needs to be put on IV fluids STAT! He is now 3% dehydrated and had 25ml of diarrhea. Calculate his fluid needs step by step using the standard 50 ml/kg/day. Calculate MR for 24 hours= Calculate HD for 24 hours= What is his CL for 24 hours= Put it all together to give me rate per hour- number. Round to the nearest whole On a standard 15 gtt/ml drip set what is his drip rate in seconds= gtt/?sec) (write as ?Two 0.9 mL aliquots of a serum are placed in two separate test tubes. These two test tubes are labeled as A and B. To tube-A, 0.1 mL of water is added. To tube-B, 0.1 mL of 200 mg/dL urea is added. Both specimens (A ad B) are analyzed for their urea concentration, and obtained following results. Tube-A= 24 mg/dL Tube-B 43 mg/dL = What is the percent recovery of this experiment? 75% 95% 58% 90%