L sample diluted 1:1 with trypan blue is counted using a hemocytometer. A total of 325 viable cells and 52 dead cells were counted in the four corner square and the big central square of the meter. What is the total number of dead cells in the original sample? * A. 2.1 x 10^6 B. 5.0 x 10^6 C. 1.1 x 10^6 D. 6.5 x 10^6

L sample diluted 1:1 with trypan blue is counted using a hemocytometer. A total of 325 viable cells and 52 dead cells were counted in the four corner square and the big central square of the meter. What is the total number of dead cells in the original sample? * A. 2.1 x 10^6 B. 5.0 x 10^6 C. 1.1 x 10^6 D. 6.5 x 10^6

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter4: Basic Immunology And Immunohematology

Section4.3: Tests For Rheumatoid Factors

Problem 2.1CS

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You are given a cell stock A. You make a 1:100 dilution and load 10µL diluted sample to a hemocytometer. After counting all 5 "one millimeter squares" you get an average of 60 cells. Then you decide to do a 1:1000 dilution to verify your counting. How many cells do you expect to get in average with 1:1000 dilution? Select one: a. 6 b. 12 с. 30 O d. 20 To do a 1:100 serial dilution. This is what you will do: Select one: a. You take 1uL of original cell stock and add into 99 uL of 1×PBS to make a 1:100 dilution b. You take 90uL of cell stock and add that into a tube containing 10uL of 1x PBS. c. You take 99uL of cell stock and add that into a tube containing 10uL of 1×PBS. d. You take 10uL of original cell stock and add into 90 uL of 1x PBS to make a 1:10 dilution. You thoroughly mix the 1:10 dilution tube. Then you take 10uL out from 1:10 tube and add it into a new tube containing 90uL of 1×PBS.
After taking an aliquot of your cells and diluting them with Trypan Blue in a 1:1 dilution, you load the cell/Trypan mixture onto a hemocytometer to count the cells. You obtain the following counts: Quadrant Transparent cells Blue cells 1 2 3 4 LO 65 5 71 68 73 2 4 1 3 67 4) If your original cell culture (the one you took an aliquot from to count) has 13 mL of cell suspension, what is the total number of cells you have in culture? Provide your answer as the full number 2
A 5mL sample diluted 1:1 with trypan blue is counted using a hemocytometer. A total of 325 viable cells and 52 dead cells were counted in the four corner square and the big central square of the meter. What is the total number of viable cells in the original sample? * A. 8.0 x 10^6 B. 3.3 x 10^7 C. 4.1 x 10^7 D. 6.5 x 10^6
You counted 4, 6, 12, 3 cells in each of the 4.outed squares of a hemacytomeyer. What are the cells per milliliter in that culture? If you resuspended your cell pellet 2.5 mL, what is the total cell count? How many uL do you need to add to a new culture if you want 4250 cells?
At the station there is a 2 mL microcentrifuge tube containing sheep blood and 3 tubes containing the following solutions: 0 mM, 300 mM, 600 mM of sucrose. The tubes are randomly labelled A, B, C. 2 mL of each solution were transferred to a different microcentrifuge tube, 20 ul of blood were then added to each tube, and they were mixed well by inverting the tube for multiple times. They were observed and it was determined whether the solution in each tube was hypotonic (Yes or No) relative to the blood cells. In addition, the solutions were diluted with water to help you determine which solution is which sucrose concentration. What sucrose concentration corresponds with each tube? Tube Hypotonic (Yes/No) Hypotonic after 1:1 dilution with water (Yes/No) Most likely Sucrose concentration (mM) A No Yes B Yes Yes C No No
Draw cells in 4 large squares of the hemocytometer showing: 48 total viable cells (hollow dots) 9 total nonviable cells (shaded dots) 2 viable cells that are not counted 3 nonviable cells that are not counted
You need to prepare medium for your culture cells. Your salt solution is supplied at a 10X concentration but needs to be 2X for use. You also need to add fetal bovine serum for a final concentration of 8%. What would you add of each for the correct final concentrations in 5 L of media?
Calculate the concentration of bacteriophage in the original culture from the following data. Be sure to include units. Show dilution factors for each test tube. Show the final dilution factor for test tube number 4. Show all your math. 0.1 ml 0.01 ml 0.001 ml 0.1 ml 1.0 ml plated 9.9 ml 9.99 ml 9.999 mi 9.9 m plaques Original Culture Test Test Test Test Tube #1 Tube #2 Tube #3 Tube #4 Dilution Factor: Concentration of Phage:
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Please note that these counts have all been done with Trypan Blue. Therefore, all cultures have been diluted two-fold (equal volume of cell suspension + equal volume of Trypan Blue). Take this into account when determining cell number. Any dilution listed in table is an additional dilution. Live and dead cells listed below represent the total number of cells in 5 large counting squares. *1 x 105 cells/ml cells were seeded into each well of the 24 well plate(s) on day 0 to begin the growth study. Plot this value at the zero time point on your graph.1These numbers represent the total number of cells counted in 5 large squares from three separate counts.
You have performed a serial dilution of an unknown sample and counted 73 CFU on a countable plate that was marked at 10^-4 dilution and you used 0.1 mL to inoculate the plate. What is the population of the original sample?
A laboratory technician performed a series of 10 fold serial dilutions shown below. he plated 0.1 mL from each dilution tube onto NA and got the follow numbers per plate dilution 1:10 1:10 1:100 1:1000 1:10000 1:100000 colony count >1000 >1000 808 303 38 3 calculate the concentration of bacteria in the undiluted stock (in CFU/mL)