A 1 mL microbial suspension comparable to 1 mL of 1.0 McFarland standard was subjected to a series of dilutions. An aliquot of 0.2 mL was transferred into a 100-mL volumetric flask and diluted with sterile saline solution to its final volume. Then a 0.1 mL of the resulting solution was transferred into a 50-mL volumetric flask diluted to its final volume. What would be the final concentration?

Introductory Chemistry: A Foundation
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ISBN:9781337399425
Author:Steven S. Zumdahl, Donald J. DeCoste
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Chapter15: Solutions
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A 1 mL microbial suspension comparable to 1 mL of 1.0 McFarland standard was subjected to a series of dilutions. An aliquot of 0.2 mL was transferred into a 100-mL volumetric flask and diluted with sterile saline solution to its final volume. Then a 0.1 mL of the resulting solution was transferred into a 50-mL volumetric flask diluted to its final volume. What would be the final concentration? 

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