9. Which of the following two enzymes is secreted from E. coli cells into the surrounding environment? A) beta-galactosidase B) beta-lactamase

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8. The enzyme known as beta-lactamase acts on:
A) lactose
B) ampicillin
C) X-Gal
D) IPTG
9. Which of the following two enzymes is secreted from E. coli cells into the surrounding environment?
A) beta-galactosidase
B) beta-lactamase
10. Which of the following is correct?
A) lacZ encodes beta-lactamase and amp' encodes beta-galactosidase
B) lacZ encodes beta-galactosidase and amp" encodes beta-lactamase
11. Can E. coli cells transformed with pGal grow on media containing ampicillin?
А) yes
В) no
12. Colonies of E. coli cells transformed with pGal will be which color on agar plates containing X-Gal?
A) white
B) blue
C) colorless
13. What is the phenotype of blue E. coli colonies?
A) Lac-
B) Lac+
C) Amps
D) Amp
14. "Satellite" colonies are best described as:
A) small colonies growing in a zone where there is no ampicillin
B) small colonies which move around a larger ampicillin-resistant colony
15. After transformation of the initial E. coli strain with pGal, any satellite colonies which are present on
the agar media containing X-Gal are what color?
A) white
B) blue
С) red
D) colorless
16. On the answer sheet, please briefly explain your answer to question 15 in no more than a couple of
sentences.
Transcribed Image Text:8. The enzyme known as beta-lactamase acts on: A) lactose B) ampicillin C) X-Gal D) IPTG 9. Which of the following two enzymes is secreted from E. coli cells into the surrounding environment? A) beta-galactosidase B) beta-lactamase 10. Which of the following is correct? A) lacZ encodes beta-lactamase and amp' encodes beta-galactosidase B) lacZ encodes beta-galactosidase and amp" encodes beta-lactamase 11. Can E. coli cells transformed with pGal grow on media containing ampicillin? А) yes В) no 12. Colonies of E. coli cells transformed with pGal will be which color on agar plates containing X-Gal? A) white B) blue C) colorless 13. What is the phenotype of blue E. coli colonies? A) Lac- B) Lac+ C) Amps D) Amp 14. "Satellite" colonies are best described as: A) small colonies growing in a zone where there is no ampicillin B) small colonies which move around a larger ampicillin-resistant colony 15. After transformation of the initial E. coli strain with pGal, any satellite colonies which are present on the agar media containing X-Gal are what color? A) white B) blue С) red D) colorless 16. On the answer sheet, please briefly explain your answer to question 15 in no more than a couple of sentences.
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