Please help me find the Michaelis-Menten and the Lineweaver-Burk plots for this. I am stuck. 4. Plot 1/Vo vs 1/[S]Double-Reciprocal (Lineweaver-Get the reciprocals of VO and [S] from the table on thBouriku) sPikotid plot 1/VO vs 1/[S]. It should look like the one below.1/vVmax = 1/Y-interceptKm = -1/X-intercept-1/KM1/V.max•Vmax = 1/y-intercept•Km = - 1/x-interceptHere is how to get EtSlope = KM/Vmax[S] = 5KM1/[S]11 (K) +max1[S] = 0.5KM= m Xmax5. ValuesFrom the plot on the previous slide calculate thefollowing:+*Kcat = Vmax/E₁ (total Enzyme concentration)•Enzyme efficiency = Kcat/Km• Make sure you plug in the right volume (50 µL) and the correct concentration 班PHZ 1 2 3 40.157 0.157 0.160 0.172 0.182 0.168Coefficit#17.-0.738R=0/150#2 R=0150 ? EnzymeT. 0.386Alewity(at 410nm)PH8 20 sec, 20 sec, 20 sec, 20 sec 20 sec 20 sec»T0.094 0.0940.112 0.112R00.125 0.119 0.114 0·112 0.116 0.110230.097 0.106 0.114 0.121 0.127 0.1320.109 0.118 0.127 0.138 0.148 0.1584 0.116 0.124 0.132 0.140 0.15 0.1595 0.110 0.116 0.124 0·133 0.141 0.1496 0.120 0.126 0.136 0.145 0.159 0.162

Enzyme kinetics play a critical role in understanding the mechanisms and efficiency of biochemical…

4. Plot 1/Vo vs 1/[S] Double-Reciprocal (Lineweaver- Get the reciprocals of VO and [S] from the table on the Burikus spibotd plot 1/VO vs 1/[S]. It should look like the one below. 1/v Vmax = 1/Y-intercept Km = -1/X-intercept -1/KM 1/V max Slope = KM/Vmax [S] = 5KM max [S] = 0.5KM 1/[S] 1 K 1(k) + f [S] max X

4. Plot 1/Vo vs 1/[S] Double-Reciprocal (Lineweaver- Get the reciprocals of VO and [S] from the table on the Burikus spibotd plot 1/VO vs 1/[S]. It should look like the one below. 1/v Vmax = 1/Y-intercept Km = -1/X-intercept -1/KM 1/V max Slope = KM/Vmax [S] = 5KM max [S] = 0.5KM 1/[S] 1 K 1(k) + f [S] max X

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter13: Artifacts And Noise In Medical Instrumentation

Section: Chapter Questions

Problem 7P

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