3. This is the standard procedure for determination of nicotine in urine. A 1.00mL sample was placed in a 12mL vial. 5-aminoquinoline was added as the internal standard (5 pg). The sample was monitored using mass spectrometry at m/z values for nicotine (84) and the internal standard (144). Given the following data determine the concentration of the two unknown samples Nicotine (ug/L) 12 Area Area 0.056 0.402 0.059 51 102 157 205 0.391 0.669 1.063 0.684 1.011 1.278 0.51 1.355 0.53 1.32 Unknown 1 Unknown 2 1.18

3. This is the standard procedure for determination of nicotine in urine. A 1.00mL sample was placed in a 12mL vial. 5-aminoquinoline was added as the internal standard (5 pg). The sample was monitored using mass spectrometry at m/z values for nicotine (84) and the internal standard (144). Given the following data determine the concentration of the two unknown samples Nicotine (ug/L) 12 Area Area 0.056 0.402 0.059 51 102 157 205 0.391 0.669 1.063 0.684 1.011 1.278 0.51 1.355 0.53 1.32 Unknown 1 Unknown 2 1.18

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter24: Introduction To Spectrochemical Methods

Section: Chapter Questions

Problem 24.28QAP

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The concentration of purified OXA-M290 is tested with a BCA assay. Serial dilutions of a bovine serum albumin (BSA) stock solution are prepared, then pipetted into a 96-well plate; each dilution of the BSA standard is tested in triplicate. Then, bicinchoninic acid and Cu2+ ions are added to all of the wells of the plate. After incubating the plate for 1 hour, a microplate reader is used to measure the absorbance of all of the wells in the plate at 560 nm. This generates the following data: BSA conc. (μg/mL), Replicate 1 Absorbance, Replicate 2 Absorbance, Replicate 3 Absorbance 40, 1.360, 1.403, 1.481 20, 0.750, 0.745, 0.810 10, 0.380, 0.344, 0.398 5, 0.198, 0.160, 0.183 2.5, 0.090, 0.100, 0.085 1.25, 0.038, 0.043, 0.051 0.625, 0.024, 0.028, 0.019 Prepare a calibration curve using these data. You can use Excel, R, SPSS or an equivalent graphing software. In this graph, plot absorbance (y-axis) against the concentration of the protein standard (x-axis). Calculate and plot…
If an instrument gives a response of 1240 for a standard containing 8 ppm of a substance, how much if this substance is in a sample that gives a response of 1705? Are any assumptions needed?
i) To estimate the accuracy and precision of the method of determination of glucose in a blood sample, blood was spiked with a 88.2 ngml1 of glucose. The amount of glucose was determined in the spiked sample giving the following results in ngml1, 83.6, 69.0, 83.4 100.0 and 76.4. a)Find the accuracy of the quality control sample. b)Find the precision of the quality control sample
From a 10-mL sample, a 1-mL aliquot was taken and diluted to 100mL. From this, a 5-mL aliquot was taken and diluted to 20mL.The final 20mL was found to have a concentration of 0.004M analyte X.What is the concentration of analyte X in the 10-mL sample?
percentage purity of an ibuprofen sample was determined by a reversed phase HPLC method as follows: Y Der ● A calibration curve of peak area versus concentration (ug/mL) of pure ibuprofen was constructed The ibuprofen sample for analysis was prepared by taking 10.0204 g of powder and dissolving it in/1Lbf distilled water (solution A). 1 ml of the latter solution was further diluted to IL with distilled water (solution B). Solution B was analysed by HPLC analysis Results The equation for the best-fit line of the calibration curve is: y=26820x-1323.8. ● The average peak area for the ibuprofen sample (solution B) was 266796.2 Calculate the percentage purity of the ibuprofen powder.
In a volumetric determination of an analyte A, the data obtained and their standard deviations are as follows: Initial buret reading 0.19 mL 0.02 mL Final buret reading 7.86 mL 0.03 mL Sample mass 63.0 mg 0.2 mg From the data, find the coefficient of variation of the final result for the %A that is obtained by using the equation that follows and assuming there is no uncertainty in the equivalent mass. %A= titrant volume x equivalent mass x 100 %/ sample mass Coefficient of variation = Submit Answer % Try Another Version 10 item attempts remaining
Ooldtion Table 1. Measurements for standardization of NaOH Trial 1 Trial 2 Trial 3 1] Tared mass of KHC3H4O4 (9) 0.374 0.325 0.357 12] Burette reading of NaOH, initial (mL) 4.59 3.61 4.03 [3] Burette reading of NaOH, final (mL) 22.03 18.77 21.17 Part B. Molar Concentration of an Acid Solution Enter the number corresponding to the type of acid you have 1. HA 3 2. H2A 3. H3A Enter the unknown number. 21 Table 2. Measurements for titration of unknown acid Trial 1 Trial 2 Trial 3 [1] Volume of acid (mL) 25.0 25.0 25.0 [2] Burette reading of NaOH, initial (mL) 2.13 2.48 3.68 [3] Burette reading of NaOH, final (mL) 24.10 24.52 25.56 Part C. Percentage by Mass of an Unknown Solid Enter the number corresponding to the type of acid you have 1. HA 3 2. H2A 3. H3A Enter the unknown number. 30 Molar mass of the unknown acid 192.14 Table 3. Measurements for titriation of solid Trial 1 Trial 2 Trial 3 [1] Mass of sample (g) 26.334 29.157 27.111 [2] Burette reading of NaOH, initial (mL) 3.21 1.48…
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Sheet 5 From the following data determine the following: Define outlier and then identify the outlier in the data. Determine if the outlier should be kept of discarded from the data. Trial 1 2 3 4 HCl (mL) 22.3 28.4 29.8 29.3 NaOH (mL) 35.2 35.1 34.9 35.0
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