3. GC analysis: Dr. Eder ran a similar Sn1 reaction in her lab: to H2O, RT Br Dr. Eder uses GC to analyze her product instead of IR, what would you expect to see in the GC graph?
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- 5. Consider the chlorination (C2, hv) of optically active (2R,3R)-2-chloro-3-methylpentane. If the dichloro fraction is analyzed by gas liquid phase chromatography (GLPC), how many peaks may be seen? How many of the compounds in the dichloro fraction show optical activity?WILL HEXANE DISSOLVE fenethylline IN GC-MS?6. Sodium chloride, β-D-fructofuranosyl α-D-glucopyranoside or sodium chloride - β-Dfructofuranosyl α-D-glucopyranoside solutions are used for dehydration of pumpkin.a) Estimate the water activity of 30% β-D-fructofuranosyl α-D-glucopyranoside solution.b) Estimate the water activity of 30% sodium chloride solution.c) Estimate the water activity of solution 20% sodium chloride and 20% β-Dfructofuranosyl α-D-glucopyranoside solution.d) What do you think about the effectivity of these solutions if the water activity of pumpkins are 0.945?
- 5. The pKas of chemicals HX and HY are 5.0 and 7.0 respectively. The pKa of carbonic acid H,CO, 3. is 6.0. If you made up an ether solution of chemicals HX and HY in a separatory funnel, and then added an aqueous solution of sodium bicarbonate NaHCO3 to that separatory funnel, would both HX and HY stay in the ether layer? Or would either or both of them transfer into the aqueous layer? If one goes into the water layer, will it be in its neutral HX/HY form, or in it's deprotonated anionic form? ilter HX: ether layer or water layer? If in the water layer, in HX or X- form? Explain HY: ether layer or water layer? Explain.Figure 1 shows a 1H NMR spectrum of a solution that was prepared by dissolving 1,3,5-trimethoxybenzene (10.22 mg) in CDCl3 (1.470 g).T What is the concentration in mol dm–3 of CHCl3 present in the CDCl3? (Density of CDCl3 = 1.500 g mL–1.)Calculate the Ksp of the following compounds, given their molar solubilities. (а) PbS, 1.64 х 10-14 м (b) Cu(ОН)2, 1.76 х 10 -7 М (с) Саз(РОд)2, 1.14 х 10-7 м Submit Answer Retry Entire Group No more group attempts remain
- You want to use acid-base extraction to separate compound A (pKa = 4.9) from compound B (pKa = 9.5). You can add either NH4CI solution or NaHCO3 solution to act as your aqueous %3D layer. Which solution should you choose and why would the other one not work? You may look up any relevant pKa values.3. You performed an experiment in order to determine the quantitative analysis of total aldehyde content in 5 g of Lemon oil. During the titration step, you used 2 mL of 0.5 N KOH. Calculate the total aldehyde content of the oil over citral (MW citral : 152, f 0.5 N KOH = 1, MW KOH: 56.1, MW HCL: 36.5)Calculate k, the degradation rate constant, responsible for drug degradation in this solution (in month-1).
- Whagt is the purpose of the following? Separate the active ingredients in an unknown over the counter analgesic using TLC (thin layer chromatography). Make a preliminary identification of each active ingredient in an unknown tablet by comparing its Rf value(s) with the Rf values of four different known active ingredients:BocHN, Surya was leaving the lab in a hurry and forgot to note which unknown he added. From the reaction scheme below and the GC-MS. Help Surya figure out whether he added the chlorinated compound, or the brominated compound to his reaction. Label the base peak and molecular ion peak. CH, NH₂ Br or Cl ♦ Relative intensity 100- 80- 60- 40- 8 Acetonitrile Water (9:1) 6h 100 BocHN, CH₂ 150 m/z 200 TFA DCM (19) 1h 250 H₂N.What would your main concern be if you accidentally used wash buffer (10mM imidazole) instead of elution buffer (250mMimidazole) when trying to elute GST-DHFR-His from the IMAC column? O Low yield OLoss of the His tag Decreased purity