Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
![27. Given the following plasmid map, list how many fragments would exist and their bp length when treated with
Hindll and Nde1
Dail 91
Ben BI 51
EstAPI 179
BemBI 2683
Ndel 183
Kasl - Narl - Sfol 235
Bgll 245
Fspl 256
Pul 276
EcoO1091 2674
Aatll - Zral 2617
BiM 2542
Sspl 2501
Prull 306
Benrl 364
Acll 2297
BæAl 387
Apol - EeoRI 396
Xmnl 2294
lacza
haall - Sacl - Ecos3KI 402
Accbs1 - Kpnl 400
Awal- BsoN - Smal-
TapM I- Ymal 412
Beal 2215
MCS
Scal 2177
haml 417
Xhal 0
Pvul 2066
Accl - Hinl - Sall 29
pl. Alau a
sb 434
Avall 2059
PUC19
2,686 bp
BerDI 1985
Sphi 441
Hindi 447
Acll 1924.
Fspl 1919
Prull 628
Avall 1837
Til 641
EsaXI 659
me AllI 1822
Rgll 1813
Bpal 1784
BSPOI - Sapl 683
BsrFl 1779
Bsal 1766
Thl 781
AII - Peil 806
BerDI 1753
Dal 908
Bmrl 1744
ori
Aldl 1694
BkiM 1015
BseYl 1110
AlwNI 1217
BeeN 1292
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