20-22. TBX5 is a gene found in vertebrates which is expressed in the heart and forelimbs during development. Parts of the first and last coding exons of the human TBX5 gene are shown below, with start and stop codons in bold: Partial first coding exon: 5' -ACCATGGCCGACGCAGACGAGG-3' Partial last exon: 5' -GTGGAGCGACAATAGCTAAAGT-3' 20. Which primer pair would you use to amplify full length TBX5 coding sequence? a. 5'-ATGGCCGACGCAGACGAGG-3'and 5'-GTGGAGCGACAATAGCTAA-3' b. 5'-ATGGCcCGACGCAGACGAGG-3'and 5'-TTAGCTATTGTCGCTCCAC-3' c. 5'-ATGGCCGACGCAGACGAGG-3'and 5'-AATCGATAACAGCGAGGTG-3' d. 5'-CCTCGTCTGCGTCGGCCAT-3'and 5'-GTGGAGCGACAATAGCTAA-3' e. 5'-GGAGCAGACGCAGCCGGTA-3'and 5'-GTGGAGCGACAATAGCTAA-3' 21. What are the first five amino acids of the human TBX5 protein? Second Letter Phe UCU UCC UCA UCG UGU Cys U UGC UGA Stop A UGG UUU UAU UAC UAA UAG Tyr U UUC UUA UUG Ser a. N-Thr-Met-Ala-Asp-Ala . Stop Stop Leu Trp G CUU C Cuc CUA CUG CU Leu cCC CCA CCG CAU CÁC CAA CAG His CGU CGC CGA b. N-Met-Ala-Asp-Ala-Asp ... Pro Arg c. C-Thr-Met-Ala-Asp-Ala . Gin 1st Зrd ... d. C-Met-Ala-Asp-Ala-Asp letter Ser u letter AUU A AUC AUA AUG ACU lle ACC ACA AAU Asn Thr AAC AAA AAG AGU AGC AGA AGG e. The first five amino acids Lys Arg Met |ACG could not be determined, GUU G GUC GUA GUG GCU Val GCC GCA GCG GAU GÁC Asp GGU GGC GGA GGG insufficient information Gly c Ala GAA Glu GAG DUAC5CACUuAC

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20-22. TBX5 is a gene found in vertebrates which is expressed in the heart and forelimbs during
development. Parts of the first and last coding exons of the human TBX5 gene are shown below,
with start and stop codons in bold:
Partial first coding exon: 5' -ACCATGGCCGACGCAGACGAGG-3'
Partial last exon: 5' -GTGGAGCGACAATAGCTAAAGT-3'
20. Which primer pair would you use to amplify full length TBX5 coding sequence?
a. 5'-ATGGCCGACGCAGACGAGG-3'and 5'-GTGGAGCGACAATAGCTAA-3'
b. 5'-ATGGCcCGACGCAGACGAGG-3'and 5'-TTAGCTATTGTCGCTCCAC-3'
c. 5'-ATGGCCGACGCAGACGAGG-3'and 5'-AATCGATAACAGCGAGGTG-3'
d. 5'-CCTCGTCTGCGTCGGCCAT-3'and 5'-GTGGAGCGACAATAGCTAA-3'
e. 5'-GGAGCAGACGCAGCCGGTA-3'and 5'-GTGGAGCGACAATAGCTAA-3'
21. What are the first five amino acids of the
human TBX5 protein?
Second Letter
Phe UCU
UCC
UCA
UCG
UGU Cys U
UGC
UGA Stop A
UGG
UUU
UAU
UAC
UAA
UAG
Tyr
U UUC
UUA
UUG
Ser
a. N-Thr-Met-Ala-Asp-Ala .
Stop
Stop
Leu
Trp G
CUU
C Cuc
CUA
CUG
CU
Leu cCC
CCA
CCG
CAU
CÁC
CAA
CAG
His
CGU
CGC
CGA
b. N-Met-Ala-Asp-Ala-Asp
...
Pro
Arg
c. C-Thr-Met-Ala-Asp-Ala .
Gin
1st
Зrd
...
d. C-Met-Ala-Asp-Ala-Asp
letter
Ser u letter
AUU
A AUC
AUA
AUG
ACU
lle ACC
ACA
AAU
Asn
Thr AAC
AAA
AAG
AGU
AGC
AGA
AGG
e. The first five amino acids
Lys
Arg
Met |ACG
could not be determined,
GUU
G GUC
GUA
GUG
GCU
Val GCC
GCA
GCG
GAU
GÁC
Asp
GGU
GGC
GGA
GGG
insufficient information
Gly c
Ala
GAA
Glu
GAG
DUAC5CACUuAC
Transcribed Image Text:20-22. TBX5 is a gene found in vertebrates which is expressed in the heart and forelimbs during development. Parts of the first and last coding exons of the human TBX5 gene are shown below, with start and stop codons in bold: Partial first coding exon: 5' -ACCATGGCCGACGCAGACGAGG-3' Partial last exon: 5' -GTGGAGCGACAATAGCTAAAGT-3' 20. Which primer pair would you use to amplify full length TBX5 coding sequence? a. 5'-ATGGCCGACGCAGACGAGG-3'and 5'-GTGGAGCGACAATAGCTAA-3' b. 5'-ATGGCcCGACGCAGACGAGG-3'and 5'-TTAGCTATTGTCGCTCCAC-3' c. 5'-ATGGCCGACGCAGACGAGG-3'and 5'-AATCGATAACAGCGAGGTG-3' d. 5'-CCTCGTCTGCGTCGGCCAT-3'and 5'-GTGGAGCGACAATAGCTAA-3' e. 5'-GGAGCAGACGCAGCCGGTA-3'and 5'-GTGGAGCGACAATAGCTAA-3' 21. What are the first five amino acids of the human TBX5 protein? Second Letter Phe UCU UCC UCA UCG UGU Cys U UGC UGA Stop A UGG UUU UAU UAC UAA UAG Tyr U UUC UUA UUG Ser a. N-Thr-Met-Ala-Asp-Ala . Stop Stop Leu Trp G CUU C Cuc CUA CUG CU Leu cCC CCA CCG CAU CÁC CAA CAG His CGU CGC CGA b. N-Met-Ala-Asp-Ala-Asp ... Pro Arg c. C-Thr-Met-Ala-Asp-Ala . Gin 1st Зrd ... d. C-Met-Ala-Asp-Ala-Asp letter Ser u letter AUU A AUC AUA AUG ACU lle ACC ACA AAU Asn Thr AAC AAA AAG AGU AGC AGA AGG e. The first five amino acids Lys Arg Met |ACG could not be determined, GUU G GUC GUA GUG GCU Val GCC GCA GCG GAU GÁC Asp GGU GGC GGA GGG insufficient information Gly c Ala GAA Glu GAG DUAC5CACUuAC
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