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- You are trying to come up with a drug to inhibit the activity of an enzyme thought to have a role in liver disease. In the laboratory the enzyme was shown to have a Km of 1.0 x 10-6 M and Vmax of 0.1 micromoles/min.mg measured at room temperature. You developed a competitive inhibitor. In the presence of 5.0 x 10-5 M inhibitor, the apparent Km of the enzyme was found to be 1.5 x 10-5 M. What is the Ki of the inhibitor?If the enzyme lactase has a Vo of 0.111111111111 mM per minute when [S] = 1.0 mM, and a Vo of 0.20 mM per minute when [S] = 5.0 mM, what is its Km? Calculate Vmax of the above enzyme (lactase). Calculate the slope on a Lineweaver-Burk plot (Km/Kmax) for the above enzyme (lactase).A drug is infused continuously intravenously (IV) for treatment of a particular disease. The volume of the body fluid V = 16 liters. The drug is excreted (removed) through the kidneys at rate ke , proportional to the concentration of the drug in the body fluids. The drug is ineffective and not used by the body if its concentration is below Cmin and becomes poisonous if the concentration goes over Cmax . It is metabolized at a rate km as the concentration is >=Cmin. Let the parameter values Cmin= 4 mg/l, Cmax =40 mg/l, ke = 0.2/hr and km = 0.3/hr.a. How much is the maximum possible rate of infusion of the drug?b. How long the drug would continue to be effective if the infusion has been stopped as the concentration reached at the maximum level?
- A drug is infused continuously intravenously (IV) for treatment of a particular disease. The volume of the body fluid V = 16 liters. The drug is excreted (removed) through the kidneys at rate ke, proportional to the concentration of the drug in the body fluids. The drug is ineffective and not used by the body if its concentration is below Cmin and becomes poisonous if the concentration goes over Cmax. It is metabolized at a rate km as the concentration is 2Cmin- Let the parameter values Cmin = 4 mg/I, Cmax =40 mg/I, ke = 0.2/hr and km = 0.3/hr. a. How much is the maximum possible rate of infusion of the drug? b. How long the drug would continue to be effective if the infusion has been stopped as the concentration reached at the maximum level?Nitric oxide reacts with hydrogen to release large amounts of chemiluminescence with the characteristics of the highly cytotoxic species. The following data were measured for the reaction of nitric oxide with hydrogen: Data number 1 2 3 2 NO(g) + 2 H₂(g) → N₂(g) + 2 H₂O(g) [NO] (M) 0.1 0.1 0.2 [H₂] (M) 0.1 0.2 0.1 Based on the data, (a) Determine the rate law for this reaction. (b) Calculate the rate constant. (c) Calculate the rate when [NO] = 0.050 M and [H₂] = 0.150 M Initial rate (M/S) 1.23 x 10-³ 2.46 x 10-³ 4.92 x 10-³The active form of the enzyme alcohol dehydrogenase exists as a homotetramer (MW = 150 kDa), and has one identical active site located on each of its four subunits. You have performed a series of enzyme kinetic experiments using 0.025 mL of an enzyme sample that is 4.0 pg/mL and determined that the following Vmax = 2.442 x 10-2umoles/min What is the turnover number of this enzyme (in units of #/sec)? 76/ sec 305 / sec 152 / sec 4579/ sec 1526 / sec O O O O
- The plot below shows the concentration of reactant A during a chemical reaction (A (ag) + B(ag) C (aq) + D (aq)) as a function of time. This reaction is taking place at 310 K A and B are aqueous solutes Estimate the instantaneous rate at 80 seconds. Give your answer with units of M/s. 0.50 (A) (M) 0.45 0.40 0.35 0.30 0.25 0.20 0.15 0.10 005 0.00 0 20 Type your answer. 40 60 time (s) 80 100 120 140ive. ive. ive. 6) is al number incur 10. If x is amount of adsorbate and m is amount of adsorbent, which of the following relations is not related to adsorption process? (a) x/m = f (p) at constant T (b) x/m = f(T) at constant p (c) p = f(T) at constant (x/m) (d) = pxT m TOKOLL Postion of (a) polar inner (b) polar surface (c) distribu (d) are preIn a study of the conversion of methyl isonitrile to acetonitrile in the gas phase at 250 °C CH3NC(g)CH;CN(g) the concentration of CH,NC was followed as a function of time. It was found that a graph of In[CH3NC] versus time in seconds gave a straight line with a slope of -4.77x10-3 s1 and a y-intercept of -3.86 Based on this plot, the reaction is v order in CH,NC and the rate constant for the reaction is -1 zero first second
- A continuous source has been leaking contaminant for 2 years into a 1-D aquifer. The steady state dilution attenuation factor DAF for the aquifer is 100. DAF = Concentra- tion at source/concentration at receptor well. Source concentration 1200 mg/L (a) What is the concentration of benzene at a receptor well after 2 years. (b) What is the concentration of benzene at a receptor well in 2 years, if there is first order decay at a rate of 0.01 (1/day).What is the mole fraction of magnesium nitrate, Mg(NO3)2, in an aqueous solution that is 5.549 wt % in magnesium nitrate? MW(Mg(NO3)2) = 148.315 g/mol. 5.834e-2 1.721e-1 7.080e-3 9.929e-1Solve the given problem related to population growth. The number of bacteria N(t) present in a culture at time t hours is given by N(t) = 2000(2). Find the number of bacteria when t = 0 hours and t = 5 hours. (a) t = 0 hours (b) bacteria t = 5 hours bacteria