ABSTRACT
This report presents the physiological changes in the heart rate of a fresh water crustacean Daphnia magna when exposed to caffeine and alcohol. Different Daphnia magnas were placed in a depression slide containing fresh water and later exposed to solutions of caffeine and alcohol. Each Daphnia magna had different responses. These data suggest there is an increase in heart rate when a Daphnia magna is exposed to caffeine and a decrease in heart rate when it is in a solution of alcohol.
LAB REPORT
Title: Physiological Changes in the Heart Rate of Daphnia magna When Exposed to Stimulants (Caffeine and Alcohol).
Prepared for: Professor Samantha Snavely
By: Carolyn Omar Iduh
June 27, 2015
Introduction The inverse effects between the consumption of stimulant like caffeine and depressant like alcohol has been well established over the years. Alcohol has a paradoxical effect that makes it first appear as a stimulant (American heart association, 2015). The two faced nature is due to ethanol the most active ingredient in alcohol. Alcohol slows down the nervous system by acting on the brain’s inhibitory neurotransmitter called gamma-aminobutyric acid. Studies have shown Bradycardia (slow or decreased heart rate) in people who consume too much alcohol (ehealthMe, 2015). Drinking alcohol can completely alter a person’s concentration, mood, and coordination (National Institute on Alcohol Abuse and Alcoholism, 2013). Too much consumption of alcohol could
Not only does this paper discuss the affect caffeine has on BP and heart rate, but it also goes into great depth on the other affects caffeine can have on the human body. While this source provides one with a lot of information, we would mainly be using the information on the cardiovascular effects and possibly behavior. This source can also be used in order to find additional sources because it discusses many results from previous studies and trials.
After completing the experiment we found that when we gave the Daphnia caffeine the heartbeat rate did show an increase. However, we also found that alcohol also increased the number of times the heart beat. Even though we performed all of the experiments very carefully, we cannot be certain that the effect we saw was due to the drugs. Perhaps the change in heartbeat rate is caused by
The following was the procedure used by the team that introduced chemicals into the environment of the Daphnia. First a zero reading was taken before any chemicals were introduced. The zero reading was an observation of the Daphnia’s heart rate before any substances were administered. All fluids were drawn off the slide using the corner of a Kimwipe. Then two drops of two percent alcohol solution were dropped onto the Daphnia. After a minute a heart rate reading was taken. The same procedure, including using the Kimwipe to draw off previous solution, was then used with four, six, eight, and ten percent solutions. A heart rate reading was taken after each solution was introduced.
The effects of caffeine and alcohol on daphnia are expressive of whether these substances are harmful or beneficial to the organism. By understanding the results of this experiment, it may also be understood how these substances effect humans. In this study, one daphnia was exposed to increasing levels of alcohol, while the other was exposed to increasing levels of caffeine, each in order to test the hypothesis that when given amounts of caffeine and alcohol, the daphnia will be affected the same way a human would. The effect of each substance was measured by the daphnia’s heart rate one minute after the substance was added. Results reveal that alcohol slows the heart rate, while caffeine increases heart rate. Furthermore, caffeine shows a
The barnacles were tested using a small cup filled with water which has a small rock with barnacles on it. Before the experiment was conducted, the barnacles were fed food. The change in cirri beats over one minute was determined. The average decrease of cirri beats per minute was 12.6. Although the manipulated trial was inconsistent, it is still logical to conclude that the
Common testing conducted by researchers uses many features of Daphnia. The transparency of Daphnia and the visibility of their hearts is the basis for the majority of experiments conducted on Daphnia. (Villegas-Navarro, Roses-L & Reyes, 2003). Many other researchers have conducted experiments on Daphnia while also paying attention to the cardiovascular region of Daphnia (Campbell & Matthews, 2004). Researchers have
The purpose of this experiment was to test the affect of caffeine on the heart rate of Daphnia by observing their behavior and heart rate under a microscope when exposed to different concentrations of caffeine. Caffeine is a stimulant drug used in many energy drinks and causes large amounts of stimulatory neurotransmitters to be released. Therefore, as caffeine is a stimulant drug it was hypothesized that the Daphnia heart rate would increase immensely. Daphnia is a group of microscopic, planktonic crustaceans that are arthropods that measure 1-5 millimeters in length. Daphnia live in multiple aquatic environments such as freshwater lakes, ponds and rivers. “The development of genomic infrastructure coupled with a wide range of phenotypic diversity make Daphnia a versatile model
Materials and Methods: The following procedure has been derived from the Plant Defenses Lab used by Blanar in 2018, and modified with the deviation of the tested plant defensive compounds of coffee and kava to green tea and black tea. In addition, a few of the steps were adjusted in order to fit within the parameters of the experiment. A plastic pipette was used to collect nine Daphnia magna specimens from the larger container of Daphnia magna.
In this experiment we find how caffeine can affect the heart rate of a culture Daphnia. Heart rate of a living organism’s can vary depending on the individual, age, body size, heart conditions, medication use and even temperature. This report will examine if the caffeine is good or bad for the living organism’s health and body. And discuss about where the caffeine is produced and used in daily life of human beings and on the environment. Daphnia is a water flea used in this experiment because of its genomic infrastructure with wide range of phenotypic diversity. This quality of Daphnia makes them a versatile model for the experiment. Also their transparent body allows the experimenter to visually see how the heart beats and count them under the light microscope during the experiment as required. The heart rate of Daphnia is monitored under different concentration of caffeine solution and the results are shown in a table and a graph. Experiment carried out to locate the effects of caffeine on a heart rate of Daphnia may or may not be a predictor of change in human heart rate under caffeine. The effects of caffeine can also be tested on humans but those experiment involving humans contains high risk, as Daphnia can only live for a short period of time and in nature most of them get eaten within their first few days or weeks of life.
Investigating the Effect of Alcohol on Heartbeat of Daphnia Daphnia are the organisms that are involved in this experiment to find out what effect alcohol has on their heartbeat. It is easy to study the effects of alcohol on the heart of Daphnia as the organ can be easily seen through the transparent body of Daphnia. The number of heartbeats may be counted before submersion in alcohol and after submersion in alcohol to investigate the effect of alcohol. Daphnia belong to the Phylum Arthropoda and are Branchiopoda which belong to the class, Crustacea. Daphnia are invertebrates and also have an exoskeleton, jointed appendages, a dorsal heart and open blood system.
The development of A. salina will be unfavorable if exposed to too much ethyl alcohol. Treatment two and three have more ethanol alcohol than treatment one and more brine shrimp died when put into more ethanol alcohol. Since the viability was less than the viability in treatment one, the hypothesis was supported. Treatment four was the constant and had no ethanol alcohol which less cysts died when compared to treatment two and three, but when compared to treatment one, more had died,
The experiment took place in a laboratory setting, and the first step was obtaining sixty individual Daphnia magna (that were neither adults nor tiny offspring) from a large tank in the lab. These individuals were equally divided into three groups; low density, medium density, and high density. The twenty Daphnia assigned to the low density group were split into four groups of five and pipetted into one of four tubes filled with 10mL of Chlamydomonas algae. The twenty Daphnia assigned to the medium density group were split into two groups of ten and placed into one of two tubes also filled up to 10mL with Chlamydomonas. The final twenty Daphnia were all placed into a single tube filled with 10mL of the algae. In order to avoid suffocation-related
Daphnia are used to test water toxicity, they are a vital part of fresh water ecosystems. They are a food source for smaller fish and invertebrates and considered to be a consumer of algae and bacteria (Elbert, 2005). These small crustaceans range in size from 2-5mm long and are commonly referred to as water fleas. Daphnia belong to the group called Daphniidae, which is a relative of freshwater shrimp. It is easy to see the internal organs of daphnia because of a transparent taco shell like carapace. A carapace is the hard outer part of a shell or covering. A daphnia’s life span can range between 1-56 days. (Elenbaas, 2013; Clare 2002). The comfortable pH level for daphnia is between 7.2 and 8.5 (Clare, 2002).
The purpose of this investigation is to test the effect of differing pH levels on the heart rate of bivalve mollusks.
An investigation into the effects of varying seawater concentrations on two marine invertebrates’ osmoregulatory abilities; Carcinus maenas and Arenicola marina.