E7.05.1 Lethal Dose Response Lab.docx

E7.05.1 Lethal Dose Response Lab Report Instructions: In this lab activity, you will examine data in order to create a dose-response curve and determine the dose that is lethal to 50 percent of the population. You will be searching for a specific dose response, and you will have limited sets of data to achieve your goal. Submit your lab report to your instructor when completed. You can find this lab on Page 6 of Lesson 7.05. Be sure to review the grading rubric below for the lab activity before submitting both items to your instructor. Use the attached Student Example to review how to complete a lab report. Title: Objective(s): See slide 1 of virtual lab for these objectives. List the objectives below.  identify the primary principle in toxicology.  describe events, pollutants, and effects on human health.  model, simulate, and evaluate dose-response relationships. Variables: The variables are described in detail your virtual lab activity. Read these descriptions in the virtual lab to determine which part of a dose-response relationship is the independent variable and what part is the dependent variable. Independent Variable: Dose: the amount of a substance that enters a body, usually reported in comparison to body mass. Dependent Variable: Response: the amount and type of damage caused by the exposure to a chemical substance. Hypothesis: Complete the predictions below.

CVA AP Environmental Science – E7.05.1 Lethal Dose Response Lab Prediction 1 : If we increase the dose of the chemical compound, the lethal response will exponentially increase, then decrease. Prediction 2 : If a different drug is tested and found to have an LD 50 level of 5mg/kg, this drug will be more dangerous than Drug X. Prediction 3 : Rank the following compounds from 1–4 regarding which substance you predict would be the most lethal based on your current body mass, with 1 representing the highest LD 50 and 4 representing the lowest LD 50 .  Nicotine - 1  Alcohol - 2  Sodium Chloride - 4  Sucrose - 3 Procedure: Access the virtual lab and complete the procedures described. Provide a brief summary of the steps you followed during the experimental procedure. Materials: Lethal Dose Response Virtual Lab Data and Calculations: Table 1: Sodium Cyanide (Sample size = 190) Dose Number of Deaths Death Rate (%) 1 8 4.2% 2 16 8.4% 3 30 15.8% 4 48 25.3% 5 68 35.8% 6 89 46.8% 7 119 62.6% 8 140 73.7%

CVA AP Environmental Science – E7.05.1 Lethal Dose Response Lab 9 173 91.1% 10 190 100.0% Graph Table 1: Take a screenshot of your graph for Table 1. Place the graph below. As an alternative, create graphs of your data using the insert chart function of the lab report document. Table 2: Arsenic (Sample size = 210) Dose Number of Deaths Death Rate (%) 2 2 0.1% 4 6 2.9% 6 11 5.2% 8 22 10.5% 10 41 19.5% 12 72 34.3% 14 131 62.4% 16 198 94.3% 18 210 100.0%

CVA AP Environmental Science – E7.05.1 Lethal Dose Response Lab Graph Table 2: Take a screenshot of your graph for Table 2. Place the graph below. As an alternative, create graphs of your data using the insert chart function of the lab report document. Table 3: Lethal Human Dose Formula to calculate lethal human dose: Substance LD 50 (mg/kg) Lethal Human Dose Sucrose (table sugar) 29,700 1782 doses Ethanol 7,060 423.6 doses Sodium Chloride (table salt) 3,000 180 doses THC (found in cannabis) 1,270 (males) 730 (females) 76.2 doses Aspirin 200 12 doses Caffeine 192 11.52 doses Nicotine 50 3 doses Arsenic 14 0.84 dose Sodium Cyanide 6.4 0.384 dose Venom of the Australian Inland Taipan Snake 0.025 0.0015 dose VX Nerve Gas 0.0023 1.38*10^4 dose

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