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23 Samples of five plant tissue discs were incubated in dilute sodium chloride solution at different temperatures. After 24 hours, it was found that the uptake of ions from the solutions was as shown in the table (arbitrary units). Comment on how absorption of sodium chloride occurs, giving your reasons. Sodium ions Chloride ions Tissue at 5°C 80 40 Tissue at 25°c 160 80

If you wanted to test the effect of a 1:500 dilution (500-fold) of coffee on PtK2 cells, how much of a 1:100 working stock of coffee would you add to 6mls in an experimental flask?  Would this be a good volume to add?  Why or why not?

Weight of potato slices during osmosis 3.5 3. 2.5 2 1.5 1 0. 0% 5% 10% 15% 20% 25% 30% Sucrose solutions 15 • 30 45 Linear (15) Linear (30) Linear (45) Q1. What information can you determine from the x-intercepts in this graph? Q2. What is the x-intercept for the 45minute time point data? Q3. What does this tell you about the solute concentration of your vegetable cells? Q4. If you cannot see the X-intercept, how could you mathematically determine this value? (hint: what is the equation of a line? How do you find the x intercept using the equation?) D Foce Weight

Measurement of beemolulose transport kinetics into oocytes in the presence of different sugars. 1) Analysis the table 2) The effect of each substrates into Km and Vmax

50 μm 10 μ A major difference between the process Image 1 could be of (a) dividing shown in the images is that and image 2 could be of (a) sea urchin egg; dividing cells in the root tip of a plant; the animal cell is forming a cell wall between the two daughter cells cells in a lily plant; dividing sea urchin egg; the plant cell has a row of fusing Golgi vesicles that will separate the two cells cells in a lily plant; dividing sea urchin egg; the animal cell has a contractile ring of actin and myosin separating the cells sand dollar egg; dividing cells in a lily plant; the plant cell does not have a ring of actin and myosin pinching the cell inward sand dollar egg; dividing cells in a lily plant; the animal cell has a row of fusing Golgi vesicles that will separate the two cells © Dr. David Phillips/Visuals Unlimited, Inc. © B. A. Palevitz, E. H. Newcomb/ Biological Photo Service

121 11) The table below compares and contrasts carrier protein pumps with clathrin-dependent receptor-mediated endocytosis. Fill in the empty spaces of the table with the appropriate answer Clathrin- Carrier protein dependent receptor mediated endocytosis pump Involvement of multiple types of proteins? (Yes/No) Involvement of ATP? (Yes/No) Involvement of dynamin? (Yes/No) Membrane proteins involved are specific for only a small number of different types of substances? (Yes / No) Possible direction of movement of transported molecules (Into cell/Out of cell/Both) Requires formation of a vesicle? (Yes/No)

19 9田 esc Response Sheet #9: Cell Division-Mitosis Name: tab Winter 2022 Bio 131 Lab Section: ps lock 1. The two major reasons that a cell undergoes mitosis is: and 2. a. What is one major difference between animal and plant cell division? it fn b. During what stage of cell division is this difference observed?

The classic demonstration that cell plasma membranes are composed ofbilayers depends on the following kinds of data:• The membrane lipids from 4.74 x 109erythrocytes will form a monolayer of area 0.89 m2when spread on a water surface.• The surface of one erythrocyte is approximately 100 μm2in area. Show that these data can be accounted for only if the erythrocyte membrane is a bilayer.

Taking into account that the diffusion constant of O2 D = 1000 um2/s, how long will it take for O2 to go from the edge of a cell of 20 um diameter to its midpoint?  A. What if the cell is 2 um in diameter? B. Taking into account that the first case is a eukaryotic cell and the second is a bacteria, which one will have the faster metabolism?

If the number of cells in a bacterial culture rises by a factor of two in one generation, and n is the duration as determined by the formula in equation 1, N is the number of people in the group (2") No way ——- (1) (a) Calculate the number of cell divisions in 4 hours assuming the bacterial cell divides every 20 minutes under normal settings; (b) Calculate the number of cells generated after 4 hours assuming the bacterial cell divides every 20 minutes under standard conditions. (c) Under the same circumstances, how long will it take to make 8192 cells? (d) Calculate the hours if one bacterium cell divides every 30 minutes.

select the correct sequential steps for restricting potency monopotent pluripotent totipotent

The classic demonstration that cell plasma membranes are composed ofbilayers depends on the following kinds of data:• The membrane lipids from 4.74 x 109 erythrocytes will form a monolayerof area 0.89 m2 when spread on a water surface.• The surface of one erythrocyte is approximately 100 μm2 in area.Show that these data can be accounted for only if the erythrocyte membraneis a bilayer.

Question 3. What molecule facilitates the bending of the ER membrane when a COPII-coated vesicle is formed on the surface of the ER?

You are growing up myoblasts C2C12 cells,to use in a myogenic study,You are using T-150 flasks with a culture area of 150 cm2 and when confluent contains 2*107 cells. you seed a culture at 8 am on Monday with 5*105 cells .Assume all survive a generation time of 18 hrs  and all cells are actively dividing.when will you expect them to be confluent?

estion 5 of 15 > Intestinal epithelial cells pump glucose into the cell against its concentration gradient using the Nat-glucose symporter. Recall that the Na+ concentration is significantly higher outside the cell than inside the cell. The symporter couples the "downhill" transport of two Na+ ions into the cell to the "uphill" transport of glucose into the cell. AGgluc If the Na+ concentration outside the cell ([Na+]out) is 143 mM and that inside the cell ([Na+]in) is 19.0 mM, and the cell potential is -53.0 mV (inside negative), calculate the maximum energy available for pumping a mole of glucose into the cell. Assume the temperature is 37 °C. = kJ mol Attempt 14 What is the maximum ratio of [glucose] in to [glucose lout that could theoretically be produced if the energy coupling were 100% efficient? 3.3 x 10-4 O 1.13 3.0 × 10³ 8.01

The function of FtsZ was determined in part by using a temperature-sensitive ftsZ mutant. What is the phenotype of an ftsZ mutant when it is grown at 37°C? Cells of random sizes (some very small) are formed after cell division Normal sized cells are formed after cell division Cells do not divide, and turn into long filaments Cells die immediately upon shifting to 42°C

Plate A = filter paper dipped into 5% solution of unknown Dye A %3D Plate B = filter paper dipped into 5% solution of unknown Dye B Both plates were incubated at 37°C. At the end of the 60 minute incubation period the diffusion rings for each dye were measured. The diffusion ring for Dye A = 40 mm. The diffusion ring for Dye B = 24 mm %3D Based on the results, which dye had the higher molecular weight? Explain your answer.

using the cell potential table found in the Reference Material,  why do you get holes in your aluminum foil if you use it to cover lasagna in a steel pan? Hint: Steel is made up of mostly iron and lasagna serves as an acidic medium.

Two co-op students at your start-up company have been asked to evaluate the rate of reaction occurring in a transparent gel particle containing immobilized mouse melanoma cells. The equation for the reaction rate ris is: Vmax T'As Km+CAs where R=3.2 mm, Vmax= 0.12 gmol s' m3, CAS = 41 gmol m3, and Km=0.8 gmol m3. One student reports a reaction rate of 1.6x10 gmol st; the other reports 1.6x10 1º gmol s1. You left your calculator on the bus this morning, but must know quickly which student is correct. Use an order-of- magnitude calculation to identify the right answer. Which student is correct?

1.2. Compare and contrast between the ATP-dependent movement employed by kinesin-1 and myosin II along their relevant cytoskeletal structure. 1.3. Monastrol is a kinesin-5 inhibitor and has the potential to be used as an anticancer agent. Discuss how this may affect mitosis. 2.1. The lipid bilayer is asymmetrical. Discuss why this is important for cellular function. Use an example to support your answer.

Calculate the amount of time it would take a cell ofEscherichia coli (1 * 2 μm) swimming at maximum speed(60 cell lengths per second) to travel all the way up a3-cm-long capillary tube containing a chemical attractant

What triggers the movement of the thin filament? Is it because of the high --> low energy configuration? The exact mechanism is confusing to me.

When a clam sperm contacts a clam egg, it triggers ionic changes that result in the breakdown of the egg nuclear envelope, condensation of chromosomes and initiation of meiosis. Two observations confirm that ionic changes initiate these cellular events:  suspending clam eggs in seawater containing 60 mM KCl triggers the same intracellular changes as do sperm suspending eggs in artificial seawater lacking calcium prevents activation by 60mM KCl. The intracellular K+ concentration is 344mM and that of normal seawater is 9 mM.   Why does 60mM KCl affect the changes mimicking the sperm cell? please explain. What does the lack of activation by 60mM KCl in calcium-free seawater suggest about the mechanism of KCl activation? please explain the mechanism.

Or. Foyez Samar The volume enclosed by the plasma membrane of plant cells is often much larger the c corresponding volume in animal cells. The most regsengble explanation for this observation Is that A) plant cells are capable of having a much higher surface-to-volume ratio than animal cells. B) plant cells have a much more highly convoluted (folded) plasma membrane than animal cens. C) plant cells contain a large vacuole that reduces the volume of the cytoplasm. D) animal cells are more spherical, whereas plant cells are elongated. 12 A u

Propidium iodide (PI) can be used to study both cell division and apoptosis using flow cytometry. Describe how PI is used to study the two cellular events with the principles explained. You are expected to give the full principle of the flow cytometric analysis of apoptosis.

Question:- where do each of thse go? which box

Using two centrifuges (A and B), with maximum speeds of 1500 rpm (r = 8 cm) and 3000 rpm (r = 8 cm), respectively, construct a separation scheme for separating three subcellular structures with the following xg requirements: PAT – 150 xg - SAT – 120,000 xg НАТ - 650 хg

Autophagy Is Required for PKA Activation and Cell Viability upon GlucoseStarvation. The functional relationship between PKA and autophagyis largely unclear. Because PKA controls cell metabolism particularly related to glucose status (21–23), we first examined the relationship between autophagy and PKA activity in the control of cellmetabolism with glucose limitation. We found that in addition toactivating autophagy, glucose starvation also induced the phosphorylation of cAMP response element-binding (p-CREB), a wellknown PKA substrate, indicative of elevated PKA activity in bothHEK293T and HCT116 cells. In contrast, disruption of autophagyby deletion of essential autophagy gene Atg7 or Atg14 abolished theincrease of p-CREB levels in the above cells (SI Appendix, Fig.S1 A, B, D, and E), suggesting that inactivation of autophagy suppresses PKA activity in response to energy shortage. Moreover, lossof Atg7 or Atg14 accelerated cell death in response to glucosestarvation (SI Appendix, Fig.…

The allosteric regulation of ATCase by CTP is an example of:   1. Negative homotropic allostery   2. Positive homotropic allostery   3. Negative heterotropic allostery   4.  Positive heterotropic allostery

A plant cell with a ΨS of -0.65 MPa maintains a constantvolume when bathed in a solution that has a ΨS of -0.30 MPaand is in an open container. The cell has a(A) ΨP of +0.65 MPa.(B) Ψ of -0.65 MPa.(C) ΨP of +0.35 MPa.(D) ΨP of 0 MPa

Only 37 of the proteins in mitochondria are encoded by the mitochondrial DNA. The other proteins are encoded in nuclear DNA, then translated and transported to the mitochondria. Describe one unique feature of protein transport across mitochondrial membranes.