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Cellular Respiration in Germinating Peas Darnell Lawrence
12/27/2023 1
Data Activity 1 Data Table 1 Sample Initial volume (V
i
) (mL) Final volume
(VF) (mL) Total volume (V
F
–
V
i ) (mL) Germinating peas 25
35
10
Dormant peas + beads 25
35
10
Beads only 25
35
10
© 2016 Carolina Biological Supply Company
2
Activity 2 Data Table 2 Water
temp. (°C) Time
(min)
Resp.
#1 Volume
in pipet
Resp.
#1 Change
in
volume
Resp.
#1 Correcte
d
volume
change
Resp.
#2 Volume
in pipet
Resp.
#2 Change
in
volume
Resp.
#2 Correcte
d
volume
change
Resp.
#3
Volum
e
in
pipet
Resp.
#3 Change
in
volume
26 0 0.22
- -
0.01
-
0.00 - 25 5 0.60
0.38 0.38 0.05
0.04
0.04
0.00 0.00
25
10 1.05
0.45 0.40 0.10
0.05
0.00
0.05 0.05
25
15 1.15
.10 0.09 0.18
0.08
0.07
0.06 0.01
25
20 1.70
0.55 0.52 0.24
0.06
0.03
0.09 0.03
1.
Which of the respirometers serves as control? Explain your answer. In this experiment, respirometers are used to measure the
rate of respiration in different conditions. The negative control
is the respirometer which only contains beads. This is because
the beads do not respire, and therefore, any change in the
oxygen levels in this respirometer can be attributed to factors
other than respiration, such as changes in atmospheric pressure
or temperature. This helps to ensure that the changes observed
in the other respirometers are indeed due to respiration.
The positive control is the respirometer containing peas. The
germination of peas involves respiration, which consumes
oxygen and produces carbon dioxide. The KOH (potassium
hydroxide) in the respirometer absorbs the carbon dioxide
produced, creating a change in pressure that draws the food
coloring down the pipet. This allows the rate of respiration to be
visually observed and measured.
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