PHGY 213 - April 2006 Final

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NAME: STUDENT NUMBER: Faculty of Science Final Examination PHYSIOLOGY 213 INTRODUCTORY PHYSIOLOGY LABORATORY 2 Version 1 Thursday, April 13, 2006 9:00 AM - 12:00 PM Examiner: Dr. J. Trujillo-Martinez Associate Examiners: Drs. D. Watt, G. Zavorsky, I. Bates, M. Guevara & Ewen Jones INSTRUCTIONS: 1. 6. 7. This examination consists of 50 multiple-choice questions (Type A and Type B). ANSWER ALL 50 MULTIPLE CHOICE QUESTIONS. All have equal value and marks are not deducted for incorrect answers. Your McGill STUDENT NUMBER (ID) and CHECK BITS (the two first letters of your LAST NAME as indicated on your ID) will be used for identification purposes in this exam. Fill in your McGill ID number on this exam paper and ALSO in the spaces marked “Student Number” ON YOUR COMPUTER ANSWER SHEET. IMPORTANT: Below the words ‘“Student Number” and “Check Bits”, FILL IN THE APPROPRIATE CIRCLES. Use a lead pencil to fill in the appropriate circle on the answer sheet. ALL ERASURES MUST BE COMPLETE. LEAVE THE COMPUTER ANSWER SHEET AND THE ENTIRE EXAMINATION as directed at the end of the exam. NO part of this examination may be taken out of the examination room. CHALLENGES / EXPLANATIONS: If you think that a given question is unclear or ambiguous, MARK THE NUMBER(S) OF THE QUESTION(S) AND THE ANSWER YOU HAVE GIVEN AT THE TOP OF THIS PAGE. Use the back of this sheet to very briefly explain the reasoning you have used to arrive at your answer. If your reasoning is correct, it will be taken into account in the determination of your grade. Challenges WILL NOT be considered after the examination has been written; therefore, please ensure that any questions and/or explanations are SUBMITTED ON THIS SHEET. SCIENTIFIC CALCULATORS (NO LANGUAGE MEMORY) ARE PERMITTED. This is a closed book exam. Translation dictionaries are allowed. WARNING: The Examination Security Monitor Program detects pairs of students with unusually similar answer patterns on multiple-choice exams. Data generated by this program can be used as admissible evidence, either to initiate or corroborate an investigation or a charge of cheating under Section 16 of the “Code of Student Conduct and Disciplinary Procedures”.

PHGY 213 2 FINAL EXAMINATION — Version 1 Thursday, April 13, 2006 TYPE A: Choose the ONE that is BEST in each case and darken the appropriate circle on the answer sheet. 1. Human chorionic gonadotropin (hCG) is composed of o and [ subunits. Which of the following statements is CORRECT regarding the subunits of hCG? a) o and 3 are glycoproteins, meaning they have carbohydrate groups attached that slow their mobility in an SDS-PAGE electrophoresis gel. b) B has a larger molecular weight than o, and will migrate faster in an SDS-PAGE electrophoresis gel. c) o and 3 are small molecules that will co-migrate at the same rate in an SDS- PAGE electrophoresis gel. d) The o and B subunits of these hormones have different molecular weights and will migrate at the same rate. e) o and f3 are glycoproteins, meaning they have carbohydrate groups attached that increase their mobility in an SDS-PAGE electrophoresis gel. 2. The rate at which proteins move through the SDS-PAGE gel depends on many factors. Identify which of the following it does NOT depend upon: a) The electric current. b) The pH of the medium. ¢) The pore size of the gel. d) The size of the protein. e) None of the above. 3. Identity the CORRECT statement about the Bradford method of protein quantification? a) The Coomassie dye binds to Arginine, Tryptophan, Histidine, Tyrosine and Phenylalanine residues and converts to the anionic form. b) The free dye in solution is in the anionic form. ¢) When the dye binds to protein it has an Absorbance maximum of 470 nm. d) The dye reflects light at 595 nm. e) None of the above.

PHGY 213 3 FINAL EXAMINATION — Version 1 Thursday, April 13, 2006 TYPE A: Choose the ONE that is BEST in each case and darken the appropriate circle on the answer sheet. 4. Why is polyacrylamide used as a matrix instead of agarose in protein separation? a) Agarose is used to separate DNA and therefore has a pore size too small for protein separation. b) Agarose gels are run horizontally and therefore, cannot be used for protein separation. ¢) Agarose can be used to separate proteins and DNA simultaneously. d) Agarose is used to separate DNA and therefore has a pore size too large for protein separation. e) None of the above. 5. Using the Bradford method of protein quantification, you make a standard curve for a known concentration of BSA. The resultant equation is y=0.0154x-0.0041. The y axis is the Absorbance and the x axis is the protein concentration in pug/ml. After a 5 times dilution of your unknown protein sample, you get an absorbance of 0.15. What is your original protein concentration in mg/ml? a) 0.1 mg/ml. b) 0.5 mg/ml. c) 0.05 mg/ml. d) 1 mg/ml. e) None of the above. 6. Beer’s law can be used to determine the concentration of a protein sample, given the extinction coefficient of the protein that is being studied. In a cuvette with a pathlength of 1 cm, a protein sample with an extinction coefficient of 0.590 L mol cm’' was measured to have an absorbance of 0.962. What is the concentration of the protein sample? a) 1.1 mol L! b) 0.613 mol L™ ¢) 0.568 mol L™ d) 0.138 mol ! e) None of the above.

PHGY 213 4 FINAL EXAMINATION — Version 1 Thursday, April 13, 2006 TYPE A: Choose the ONE that is BEST in each case and darken the appropriate circle on the answer sheet. 7. How does a spectrophotometer work? a) Monochromatic light is passed through a sample and the amount of light that reaches a detector is recorded. b) Monochromatic light is passed through a sample and the amount of light that is reflected from the sample is recorded with a detector. ¢) Monochromatic light is passed through a sample and the amount of light that is emitted from the fluorescence of the Bradford reagent is recorded. d) Both (b) and (c) are correct. e) None of the above. 8. An experimenter applied a sudden and maintained push to the ball of a subject’s foot. The subject was prepared to oppose this force and an EMG response appeared in his gastrocnemius muscle about 120 msec after the onset of the push. What is this response called? a) Electromagnetic interference. b) A functional stretch reflex. ¢) A motion artifact. d) A monosynaptic stretch reflex. e) A voluntary response. 9. Which one of the following could have contributed to motion artifacts recorded during the Human Locomotion experiment? a) Action potentials in the motor axons. b) TV transmissions. c) Muscle action potentials. d) Recording electrode half-cell potentials. e) Switch bounce in the contact-sensing mat.

PHGY 213 5 FINAL EXAMINATION - Version 1 Thursday, April 13, 2006 TYPE A: Choose the ONE that is BEST in each case and darken the appropriate circle on the answer sheet. 10. Assume that you measured the following during the Human Locomotion lab exercise: monosynaptic stretch reflex latency: 30 msec functional stretch reflex latency: 110 msec voluntary response time: 190 msec the burst of EMG activity associated with landing from a single step began 170 msec before contact the burst of EMG activity associated with landing from a single step ended 160 msec after contact Based on these results, when would signals associated with controlling landing from a single step begin to leave the cerebral cortex? a) About 170 msec before first contact of the foot. b) About 110 msec after first contact of the foot. ¢) More than 170 msec before first contact of the foot. d) At the instant of first contact of the foot. e) More than 160 msec after first contact of the foot. 11. On average, what is the preferred hopping frequency of normal subjects? a) 2.9 hops/sec b) 1.7 hops/sec c) 2.5 hops/sec d) 1.3 hops/sec e) 2.1 hops/sec 12. In the feed-forward mode of locomotor control, how does the brain predict the motor command that will be necessary to perform a desired movement? a) It uses previous experience. b) It uses segmental stretch reflexes. ¢) It uses functional stretch reflexes. d) It compares actual movement to desired movement and corrects while the movement is occurring. e) Ituses voluntary responses to the movement.

PHGY 213 6 FINAL EXAMINATION - Version 1 Thursday, April 13, 2006 TYPE A: Choose the ONE that is BEST in each case and darken the appropriate circle on the answer sheet. 13. Which of the following is true about recording the arterial blood pressure (BP) in a normal subject? a) One would expect the systolic BP to be at least 20 mm Hg higher in the right arm than in the left arm. b) One would expect the pulse pressure to be on the order of 80 mm Hg. ¢) The systolic BP is measured at the point when the Korotkoff sounds are no longer heard. d) The Korotkoff sounds tend to get louder as the cuff pressure is reduced towards the diastolic BP. ¢) None of the above. 14. Which of the following is true? a) The first part of the P-wave is generated by depolarization of the left atrium. b) There is activation nowhere in the heart during the ST-segment. c¢) The activation of the atrioventricular node starts sometime during the PR- segment. d) The activation of the right ventricular free wall precedes the activation of the left ventricular free wall. e) The right bundle branch bifurcates into the right anterior fascicle and the right posterior fascicle. 15. A patient has two different morphologies of the P-wave in lead I, with the P-wave being positive on most beats, and negative on the other beats. You conclude: a) The person has two sinus nodes. b) Electrical activity starts on each cycle from exactly the same location in the heart. ¢) The negative-going P-wave might be generated by activity originating in the sinus node. d) The negative-going P-wave might be generated by activity originating in an ectopic focus in the left atrium. e) None of the above.

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