Lab 7_Yeast Invertase Worksheet Fa 20
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San Jose State University *
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Biology
Date
Apr 28, 2024
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Name:___________________________________________ Date:______________________ Sec,on#:_________
LAB #7: Yeast Invertase Worksheet 1.
Choose 1 variable: Enzyme Concentra,on, pH, or Temperature. Fill in your Data Table in the same format as the one in your lab manual. (1 pt) 2.
Experimental variable you are tes,ng:_____________Temperature__________________ 3.
Plot your results following the direc,ons in the lab manual. Be sure to label all axes. You can use a different piece of paper/graphing paper if you like. (2 pts) Tube #
Group 5 Abs
Group 6 Abs
1A
NO E 0.003
0.002
1B
0.034
0.044
2A
NO E 0.000
0.002
2B
0.054
0.073
3A
NO E 0.002
0.003
3B
0.164
0.165
4A
NO E 0.001
0.004
4B
0.023
0.014
5A
NO E 0.002
0.028
5B
0.010
0.012
Name:___________________________________ Date:_________________ Sec,on#:_____________
4.
At which enzyme concentra,on / pH / temperature is invertase ac,vity the greatest based on your experimental results (only answer for the experiment you completed). (1 pt) For the no enzyme its the highest at O degrees and with the enzyme its the highest at 45 degrees. 5.
What was the purpose of all the steps where you boiled the samples? (1 pt) The purpose of boiling the samples was to stop then enzyme acAvity from going any further aCer it reached opAmal acAvity in the water bath 6.
What tube or tubes are your nega,ve controls? Why are they considered a nega,ve control? (2 pts) The negaAve controls were the tubes without the invertase enzyme. They are considered the negaAve control because it did not contain any invertase 7.
Why do you expect the rate of enzyme reac,on to increase as temperature increases (un,l you start to denature the enzyme)? How does heat help? (1 pt) Heat helps in the way that it helps increase velocity and kineAc energy. The rate of an enzyme reacAon increases as temperature increases, causing the enzyme to denature. Because the molecules help reach the enzymes potenAal acAvaAon energy, in increases the rate of reacAon. 2
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Related Questions
______________________________________________________________ 1. People who carry a theoretical genetic disorder (called B-disease) can be identified from a 2kb DNA sequence. People who carry this genetic disorder have a single nucleotide polymorphism that results in a change of GTATCC to GGATCC, a site that only occurs once at nucleotide number 750 in this DNA sequence. Answer the following questions based on the information provided. (a) How can you develop a simple molecular test with enzyme BamH to identify the genetic disorder? (
(b) If you have carried out the molecular test (based on the information above) on a 100 individual and found that 24 were healthy (BB) and 26 were carriers (bb); 1) What is the ratio of heterozygous?
2) Show how can you identify the three types from the agarose gel
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Viral DNA in the cytoplasm of a cell can be processed by _________________________, producing a unique dinucleotide ___________________ as a signaling molecule, which can be detected by _____________________.
Group of answer choices
TLR3 and 2'-5' cyclic AMP (2'-5'cAMP); RIG-I
CGas; 2'-5' cyclic GAMP (2'-5' cGAMP); RIG-I
TLR3 and 2'-5' cyclic AMP (2'-5'cAMP); CGas
CGas; 2'-5' cyclic GAMP (2'-5'cGAMP); STING
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Disease: Ralstonia Wilt in Tomato
Causal Agent (Scientific Name): ___________________________________________
Part(s) affected: _____________________________
Description of damage:
___________________________________________
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___________________________ makes up a very large fraction of the human genome. LINES and SINES are examples that occur hundreds of thousands of times in the genome.
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please answer asap
as its a table so answer all table ..
NOTE: MUST BE DIGITALIZED NOT HANDWRITTEN BECAUSE I AM HAVING A HARD TIME SEEING MESSY HANDWRITING
Direction: Answer the following table below. Make sure to fill the whole table below with correct answers.
Organelles
Animal/Plant/Both
Nickname (give a nickname that is related to it's function)
Explanation (explain why you chose the nickname you provide)
Cell wall
Plant
Father
Because like the function of the cell wall, father supports and protects the family
Cell membrane
Nucleus
Centrosome matrix
Peroxisome
Chromatin
Microvilli
Microtubule
Microfilament
Cytosol
Lysosome
Vesicle
Nucleolus
Nuclear Membrane
Centrioles
Cytoplasm
Rough Endoplasmic Reticulum
Smooth Endoplasmic Reticulum
Ribosome…
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_____________ a proteomic technique that uses genetically modified yeast cells to detect protein-protein and protein-DNA interactions.
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Copy and paste the link below and watch the video in Youtube and Answer the Questionshttps://www.youtube.com/watch?v=8RBs0Ghg_48
Gel electrophoresis
Questions
1. What are the chemicals and materials used in gel electrophoresis?
2. Draw a schematic diagram of a gel electrophoresis set-up3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam?
4. What is the use of ethidium bromide and why must you wear gloves when you handle it?
5. What makes the DNA fragment move towards the positive plate?
6. What is the purpose of glycerol in the sample buffer?
7. What is the use of a DNA ladder?
8. What will happen when you increase the voltage of the set-up?
9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?
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Write in explained form
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please help me with this I want to use this? Don't copy from Google?
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Please help me answer the questioncorrectly procedure is already given as a guide thank you
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Please see the attached image.
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What process is taking place in the illustration?
Supply the appropriate labels for all numbered parts of the illustration.
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Hershey and Chase were able to see whether the backteriophage moved genetic material because they used _____________ labels to attach to sulfur and phosphorus.
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_________ Techniques Permit Detection of Specific DNA Fragments and mRNAs.
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What is the major target of the anti-inflammatory drug Aspirin (salicylate)?
Edit View Insert Format Tools Table
12pt v
Paragraph v
IUAV ev T?v
...
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RNA _________ Causes Gene Inactivation by Destroying the Corresponding mRNA.
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וןווד
←
Q
Lab Report 6 worksheets 314 F22 .DOCX
File Edit View Insert Format Tools Help
A 100%
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Summary
Grades for Arysta Visser: 23 x M Uh-oh! There's a problem w X b The restriction EcoRI cleaves X + Untitled spreadsheet - Goog X
https://docs.google.com/document/d/1mKY1HIgMPRh1kRDCmX7msBF2yf07-ogT/edit
Outline
Headings you add to the document will
appear here.
Normal text
Times ...
12 + B I U
2
18. The restriction EcoRI cleaves double-stranded DNA at the sequence 5'-GAATTC-3', the
restriction enzyme HindIII cleaves at the sequence 5'-AAGCTT-3', and the restriction enzyme
BamHI cleaves at 5'GGATCC-3. An 805 bp circular plasmid is digested with each enzyme
individually and then in combination, and the resulting fragment sizes are determined by
means of electrophoresis. The results are as follows:
1
Restriction Enzyme(s)
EcoRI
BamHI
HindIII
EcoRI and BamHI
EcoRI and HindIII
BamHI and HindIII
3
Practice
====•=•€ EX
Fragment lengths (base pairs)
430 bp, 375 bp
470 bp, 335 bp
Lab Report 6…
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Answer #4 of the worksheet
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Briefly explain why tumors are believed to resemble wounded tissues that do not heal.
View Insert
Format Tools Table
12pt v
Paragraph v
B IUAv2v Tアv
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This is a paper centrifuge: (you don't need to watch the whole video, just a few
seconds to see how it works--description below is probably sufficient if you can't
get to the link)
https://www.youtube.com/watch?v=isMYGtCFljc
It is a paper disc that has supercoiled strings through two holes in the centre--like a
button. As you straighten the strings and uncoil them, the paper spins.
The paper spins at a very high rate and can separate materials in a way that is
similar to a centrifuge.
A droplet is added to the centre of the paper and it is spun. The densest objects
travel the farthest from the centre.
Assume that we are using it to perform our mitochondrial extraction, which cellular
component would travel the furthest?
nuclei
mitochondria
microvessicles
blood cells
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Mutated DNA Sequence #2 T A C G A C C T T G G C G A C G A C T …
What’s the mRNA sequence? (Circle the change)
What will be the amino acid sequence?
Will there likely be effects? What type of mutation is this? ________________________________
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Genes are composed of _________________ and are linearly arranged on _________________.
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Question is attached as picture. Please do NOT just copy and paste the answer from another website, thank you!
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Related Questions
- ______________________________________________________________ 1. People who carry a theoretical genetic disorder (called B-disease) can be identified from a 2kb DNA sequence. People who carry this genetic disorder have a single nucleotide polymorphism that results in a change of GTATCC to GGATCC, a site that only occurs once at nucleotide number 750 in this DNA sequence. Answer the following questions based on the information provided. (a) How can you develop a simple molecular test with enzyme BamH to identify the genetic disorder? ( (b) If you have carried out the molecular test (based on the information above) on a 100 individual and found that 24 were healthy (BB) and 26 were carriers (bb); 1) What is the ratio of heterozygous? 2) Show how can you identify the three types from the agarose gelarrow_forwardViral DNA in the cytoplasm of a cell can be processed by _________________________, producing a unique dinucleotide ___________________ as a signaling molecule, which can be detected by _____________________. Group of answer choices TLR3 and 2'-5' cyclic AMP (2'-5'cAMP); RIG-I CGas; 2'-5' cyclic GAMP (2'-5' cGAMP); RIG-I TLR3 and 2'-5' cyclic AMP (2'-5'cAMP); CGas CGas; 2'-5' cyclic GAMP (2'-5'cGAMP); STINGarrow_forwardDisease: Ralstonia Wilt in Tomato Causal Agent (Scientific Name): ___________________________________________ Part(s) affected: _____________________________ Description of damage: ___________________________________________arrow_forward
- ___________________________ makes up a very large fraction of the human genome. LINES and SINES are examples that occur hundreds of thousands of times in the genome.arrow_forwardplease answer asap as its a table so answer all table .. NOTE: MUST BE DIGITALIZED NOT HANDWRITTEN BECAUSE I AM HAVING A HARD TIME SEEING MESSY HANDWRITING Direction: Answer the following table below. Make sure to fill the whole table below with correct answers. Organelles Animal/Plant/Both Nickname (give a nickname that is related to it's function) Explanation (explain why you chose the nickname you provide) Cell wall Plant Father Because like the function of the cell wall, father supports and protects the family Cell membrane Nucleus Centrosome matrix Peroxisome Chromatin Microvilli Microtubule Microfilament Cytosol Lysosome Vesicle Nucleolus Nuclear Membrane Centrioles Cytoplasm Rough Endoplasmic Reticulum Smooth Endoplasmic Reticulum Ribosome…arrow_forward_____________ a proteomic technique that uses genetically modified yeast cells to detect protein-protein and protein-DNA interactions.arrow_forward
- Copy and paste the link below and watch the video in Youtube and Answer the Questionshttps://www.youtube.com/watch?v=8RBs0Ghg_48 Gel electrophoresis Questions 1. What are the chemicals and materials used in gel electrophoresis? 2. Draw a schematic diagram of a gel electrophoresis set-up3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam? 4. What is the use of ethidium bromide and why must you wear gloves when you handle it? 5. What makes the DNA fragment move towards the positive plate? 6. What is the purpose of glycerol in the sample buffer? 7. What is the use of a DNA ladder? 8. What will happen when you increase the voltage of the set-up? 9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?arrow_forwardWrite in explained formarrow_forwardplease help me with this I want to use this? Don't copy from Google?arrow_forward
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SEE MORE QUESTIONS
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Recommended textbooks for you
- Medical Terminology for Health Professions, Spira...Health & NutritionISBN:9781305634350Author:Ann Ehrlich, Carol L. Schroeder, Laura Ehrlich, Katrina A. SchroederPublisher:Cengage LearningCase Studies In Health Information ManagementBiologyISBN:9781337676908Author:SCHNERINGPublisher:Cengage
Medical Terminology for Health Professions, Spira...
Health & Nutrition
ISBN:9781305634350
Author:Ann Ehrlich, Carol L. Schroeder, Laura Ehrlich, Katrina A. Schroeder
Publisher:Cengage Learning
Case Studies In Health Information Management
Biology
ISBN:9781337676908
Author:SCHNERING
Publisher:Cengage