Lab 5

Image 1 shows raw data of gel electrophoresis. Label/annotate image 1 (lanes, ladder sizes, etc). Explain what your seeing in the gel. Use the following information and picture 2 to assist in labelling. The purpose of this gel electrophorsis is to ensure that your GOI, FAP257, is in each BAC. Protocol that was done for Gel electrophoresis: -Place tray with gel into gel box -Fill gel box with IX TAE until the gel is completely submerged -Remove the comb for wells -Load 10ul of the 1kb Gene Ruler ladder (well 1) -Contains DNA ladder, 6X TriTrack DNA, Loading Dye, and Deionized water -Load other wells -Well 2: Control -Well 3: BAC- 15M5 -Well 4: BAC-39K10 -Well 5: BAC-27N17 -Run the gel at 100V for 30 minutes or until the dye front has migrated 2/3 down the gel

3) Define gel electrophoresis, including its theory and application. Describe the steps of running gel electrophoresis using the following image. More detailed reading: https://www.sciencedirect.com/topics/medicine-and-dentistry/agar-gel-electrophoresis POWER SUPPLY CATHODE ELECTROPHORETIC BUFFER ANODE WELL SAMPLE AGAROSE GEL POWER SUPPLY CATHODE ANOCE HIGH MOLECULAR WEIGHT SPECIES LOW MOLECULAR WEIGHT ANALYTES

0.9% salt (NaCl) concentration is isotonic to red blood cells. What would happen to a patient's red blood cells if she accidently received an intravenous fluid in the hospital that was made with 0.09% salt solution Edit View Insert Format Tools Table 12pt v Paragraph v B I U AV e T?v

+ courses/_518299_1/cl/outline?customClassicLocation=%2Fwebapps%2FBb-McGraw Hill-BB5744b9beb8ccb%2Fapp%2Flink%2Finbou.... File Edit Format Tools Help Qa 11 Chapter 2 ● Exam 2 Review Sheet ● Normal ● ● ● Unsaved Chantor 7 Edits will not be automatically saved. Calibri T Save now 14 ▾ BIU A A- EEEE VE What are the important characteristics of atoms and elements? What are the different types of chemical bonds? What are the various types of chemical reactions and solutions? What determines acidity and alkalinity, and how do they relate to the pH scale? What makes carbon the fundamental element of life? What are the four types of biological macromolecules, and what are their general structures and functions?

וןווד ← Q Lab Report 6 worksheets 314 F22 .DOCX File Edit View Insert Format Tools Help A 100% ¿ Summary Grades for Arysta Visser: 23 x M Uh-oh! There's a problem w X b The restriction EcoRI cleaves X + Untitled spreadsheet - Goog X https://docs.google.com/document/d/1mKY1HIgMPRh1kRDCmX7msBF2yf07-ogT/edit Outline Headings you add to the document will appear here. Normal text Times ... 12 + B I U 2 18. The restriction EcoRI cleaves double-stranded DNA at the sequence 5'-GAATTC-3', the restriction enzyme HindIII cleaves at the sequence 5'-AAGCTT-3', and the restriction enzyme BamHI cleaves at 5'GGATCC-3. An 805 bp circular plasmid is digested with each enzyme individually and then in combination, and the resulting fragment sizes are determined by means of electrophoresis. The results are as follows: 1 Restriction Enzyme(s) EcoRI BamHI HindIII EcoRI and BamHI EcoRI and HindIII BamHI and HindIII 3 Practice ====•=•€ EX Fragment lengths (base pairs) 430 bp, 375 bp 470 bp, 335 bp Lab Report 6…

The subcostal short axis views are achieved by rotating the probe approximately ______ degrees anticlockwise from the subcostal 4-chamber view so that the scan plane is ______________ to the 4-chamber view and the POM is directed to the ___ o’clock position.

BIOTECHNOLGY Date: Name: Instructor: Section/Group:. POST-LAB QUESTIONS 1. In one or two sentences, summarize the technique of gel electrophoresis. 2. How does the process of gel electrophoresis separate DNA fragments? 3. Why is the fact that DNA has a negative charge so important in the gel electrophoresis process? Biotechnology 165

INSTRUCTIONS: Please do not copy here in Bartleby or in Google. QUESTIONS : 1. Why is it necessary to heat the lid of the test tube source? Explain.

For letter A, pls ILLUSTRATE (create an illustration or drawing) the DILUTION SERIES of the problem just like the sample on the 2nd image.  Please read the instructions carefully as I have already posted this twice and the experts just copy-pasted the answers from my first post. I don't want to waste another post question for this one. Again, I NEED AN ILLUSTRATION and not just the computation/explanation through words so I can properly visualize the problem. WILL UPVOTE if I get what I need.

pic: 3.1 Discussion Forum с Student Po... X New Tab X ftccollege.instructure.com/courses/19318/discussion_topics/259958 Log In to Canvas Cengage eTextbook + Email | Microsoft 365 3.1 Discussion Forum What is Scatter Radiation and How is it Controlled? Type here to search Instructions 1. After reading chapter 9 from your textbook, you will be able to explain scatter radiation and how it is produced as a result of the attenuation of the x-ray beam by matter. You will also recognize the factors that influence its formation and how to minimize the fog that this radiation causes on radiographs. NCCT Note: Please read the instructions provided in the following links before submitting your entry: . • Participation Rules . Online Etiquette Rules B LM 2. For your participation in the forum, answer the following questions: a. Fog on a radiograph becomes objectionable when one body part size is larger than another. Based on what you read, explain which body part will be objectionable? b. How is…

I’m kinda confused on what to study for the test and what questions are gonna be on there but this PowerPoint but I just want you to explain the microbiology screenshots to me in a way I can clearly understand, I need a tutor

Hershey and Chase were able to see whether the backteriophage moved genetic material because they used _____________ labels to attach to sulfur and phosphorus.

Assignment_12.pdf - Adobe Reader File Edit View Window Help Оpen 1 / 1 99,1% Tools Fill & Sign Comment 3. Recombinant protein is produced by a genetically engineered strain of Escherichia coli during cell growth. The recombinant protein can be considered a product of cell culture even though it is not secreted from the cells; it is synthesized in addition to normal E. coli biomass. Ammonia is used as the nitrogen source for aerobic respiration of glucose. The recombinant protein has an overall formula of CH1.5500.31N..25. The yield of biomass (excluding recombinant protein) from glucose is measured as o.48 g/g; the yield of recombinant protein from glucose is about 20% of that for cells. How much ammonia is required? What is the oxygen demand? If the biomass yield remains at 0.48 g /g, how much different are the ammonia and oxygen requirements for wild-type E. coli that is unable to synthesize recombinant protein? а. b. с. 24°C 08:39 Berawan 27/04/2022

The first photo is the procedure while the other photo is the problem that needs to be answered. Please answer correctly, and provide proper solutions. Thank youuuu

Discuss the principles and applications of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry in molecular diagnostics.

Horizontal sequence :RIVL Vertical sequence:FMK   Scoring rules: g/o = -3, g/e = -1, match or mismatch - from PAM250 substitution matrix below.  SW algorithm.    1. Complete the scoring matrix.    Scoring matrix with PAM250 scores:   R I V L F         M         K           2. Set up, initialize and complete the SW matrix.  3. Retrace, align and score alignment(s).             Use the arrows and circles for the matrix and path(s).                                                                                                                                                                                                                                                                                                                                 R I V L             F           M           K           Align and score all optimal alignments here.  PLZ  the arrows and circles for the matrix and path(s) AND SHOW ALL possible Alignment  Here the following points…

Can I get an introduction or background information about synthesis and spectrophotometric of aspirin please thank you

Tighnari is doing preliminary protein studies on redcrest, a vibrant crimson fruit found in the hot deserts of Sumeru. He extracted proteins in the given sample using phosphate buffer. The resulting crude protein extract was subjected to gel filtration chromatography and SDS-PAGE. GFC results: - Absorbance Peak A B Figure 1. Elution profiles of the mixture of protein standards (black) and crude protein extract (blue). Table 1. Components of the calibration mixture and their corresponding molecular weight. U خلفة X A Elution Volume, mL Identity B Bovine Serum Albumin B-lactoglobulinn Horse Myoglobin Crude Protein Extract Molecular Weight, Da 66000 36800 17600