100XL UCLA

.pdf

School

University of California, Los Angeles *

*We aren’t endorsed by this school

Course

100L

Subject

Biology

Date

Apr 25, 2024

Type

pdf

Pages

Uploaded by Emily_barr9000 on coursehero.com

A Name \ > ~ :’,—a‘ Date '\._4%‘ - - - ~Vr Lab Section 5 3 (=) | was present and performed this exercise (initials) | Glo Germ™ Hand Wash Education System OBSERVATIONS AND INTERPRETATIONS 1 Record the degree of hand contamination before and after washing in the table below. Use this qualitative scale for evaluation +++ means “a lot of contamination” ++ means “moderate contamination” + means "little contamination” 0 means “no contamination” 2 There is no absolute cutoff between any of these categories, and what you call “moderate contamination” might be called “little contamination” by another student. Just try to be consistent within your evaluation. T e e Palm Fingers Between Fingers Tops of Fingernails Under Fingernails Back of Hand Front of Wrist Back of Wrist QUESTIONS l What areas were most thoroughly cleaned by your washing technique? 2 What areas were most difficult for you to clean with your washing technique? SECTION 1 Fundamental Skills for the Microbiology Laboratory 19

E 3 In general, were your two hands cleaned an equal amount, or was one cleaned more than the other? What could account for any differences? 4 How do your answers to questions 1, 2, and 3 compare to your lab partner’s answers? Why might they differ? 5 Why were you instructed to have your lab partner turn the water on and off and operate the UV lamp rather than you doing these actions yourself? 6 Why might it be advisable to modify the procedure and use the UV light to check your hands prior to application of lotion and the paper towels prior to drying? 7 Using the same qualitative scale as before, record the amount of Glo Germ™ that was transferred to this data sheet, your table top, and to your writing instrument. What does this tell you about the ease of transferring the “unseen” by contact. 20 MICROBIOLOGY: Laboratory Theory & Application, Brief

\\ N '\ ame \ I ~ ‘\.’-“‘_ ! ) Date — o - - .3 ~Va | Lab Section < \ .’\.“’ =B ! L | was present and performed this exercise (initials) ( S > | A Comparison of Hand-Cleansing Agents OBSERVATIONS AND INTERPRETATIONS 1 Record your results in the table below. For relative amount of growth, use this qualitative scale for evaluation: +++ means “a lot of contamination” ++ means “moderate contamination” + means “little contamination” 0 means “no contamination” 2 There is no absolute cutoff between any of these categories. What you call “moderate contamination” might be called “little contamination” by another student. Just try to be consistent within your evaluation. For diversity, look for different colors, shapes, textures, sizes, and anything else that is indicative of a different organism growing, and record the number of different organisms present. Interpretation would be relative effectiveness of the agent (excellent, good, fair, poor). T T T efors Afte n Plat | RO Saiailiobosinen S i S S SR | : 1 2 i | QUESTIONS ] Which agent seemed to be most effective at removing microbes from the fingertips? 2 Which agent seemed to be least effective at removing microbes from the fingertips? SECTION 1 Fundamental Skills for the Microbiology Laboratory 23 RO RN TSR

Name Date Lab Section | was present and performed this exercise (initials) Streak Plate Methods of Isolation RESULTS AND INTERPRETATIONS ] Using your pencil, perform a quadrant streak on the “practice plate” below. 2 Examine the quadrant streak and T-streak plates. Have your lab partner write a critique of your isolation technique in the space below. The following should be addressed: Was isolation produced on one or both plates? On the quadrant streak, were the first three streaks near the edges of the plate? On both plates, did any streaks intersect streaks they should not have? Was the whole surface of the agar used? Was the agar cut by the loop? SECTION 1 Fundamental Skills for the Microbiology Laboratory 51

D QUESTIONS l Did you get growth onlin the sterile NB and NA slant tubes you practiced with? If not, congratulations! If so, where did you see it and what might have been its source(s)? 2 Considering the cultures used to inoculate each mediwm in this exercise, how many different microbial types should you expect to see onlin each medium? Explain your answer. 3 Which medium was most difficult for you to transfer from? Which medium was most difficult for you to inoculate? Explain your difficulties. (Note: There are no correct answers to these questions. They are based on evaluation of your personal experience.) 4 Did you notice a difference in density (turbidity) of growth in NB tubes inoculated from NB and NA slants? Suggest possible reasons why a difference might occur. 5 Did you notice a difference in density of growth on NA slants inoculated from NA slants and NB? Suggest possible reasons why a difference might occur. a4 MICROBIOLOGY: Laboratory Theory & Application, Brief

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version