Spectrophotometry

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Dallas County Community College *

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1406

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Biology

Date

Apr 25, 2024

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docx

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3

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1 Spectrophotometry: Absorption Spectrum of Pigments Jessi Fuentes BIOL-1406-22007 Professor Birmingham March 22, 2024
2 350 400 450 500 550 600 650 700 750 -1.2 -1 -0.8 -0.6 -0.4 -0.2 0 0.2 0.4 0.6 Absorption Spectrum of Pigments Chlorophyll a Chlorophyll b Xanthophyll Carotene Total Pigment WaveLength Absorbance Value Fig 1.1- Line Graph of Absorbance Values of Pigments Wavelength(λ) Chlorophyll a Chlorophyll b Xanthophyll Carotene Total Pigment 400 0.09 0.32 0.17 0.5 0.5 420 0.12 0.37 0.33 0.32 0.48 440 0.12 0.31 0.26 0.33 0.44 460 0.07 0.23 0.13 -1 0.37 480 0.05 0.12 0.15 -1 0.29 500 0.03 0.04 0.11 -1 0.21 520 0.02 0.02 0.1 -1 0.21 540 0.02 0.11 0.14 -0.03 0.16 560 0.02 0.03 0.15 -0.08 0.15 580 0.02 0.03 0.2 -1 0.14 600 0.02 0.04 0.16 -1 0.13 620 0.03 0.06 0.14 -1 0.12 640 0.03 0.08 0.14 -1 0.12 660 0.07 0.17 0.13 -1 0.16 680 0.03 0.18 0.16 -1 0.12 700 0.02 0.02 0.11 -1 0.08 720 0.01 0.02 0.26 -1 0.07 Fig 1.2 Data Table of Results of Chlorophyll a, Chlorophyll b, Xanthophyll, Carotene, and Total Pigment
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