AP_Bio_Lab_8_Bacterial_Transformation

I need help writting a biological "title" for this lab report. I performed 5 labs which are PCR purification, Rapid ligation, and Transformation, Blue/White screening and observation of pGreen transformation, Plasmid Extraction and Restriction Enzyme Digestion, Soil DNA extraction and gel electrophoresis, and Bioinformatics Analysis of 16s rRNA genes.   Note; I attached one page of my abstract and one page of the introduction

BIOTECHNOLGY Date: Name: Instructor: Section/Group:. POST-LAB QUESTIONS 1. In one or two sentences, summarize the technique of gel electrophoresis. 2. How does the process of gel electrophoresis separate DNA fragments? 3. Why is the fact that DNA has a negative charge so important in the gel electrophoresis process? Biotechnology 165

Assignment_12.pdf - Adobe Reader File Edit View Window Help Оpen 1 / 1 99,1% Tools Fill & Sign Comment 3. Recombinant protein is produced by a genetically engineered strain of Escherichia coli during cell growth. The recombinant protein can be considered a product of cell culture even though it is not secreted from the cells; it is synthesized in addition to normal E. coli biomass. Ammonia is used as the nitrogen source for aerobic respiration of glucose. The recombinant protein has an overall formula of CH1.5500.31N..25. The yield of biomass (excluding recombinant protein) from glucose is measured as o.48 g/g; the yield of recombinant protein from glucose is about 20% of that for cells. How much ammonia is required? What is the oxygen demand? If the biomass yield remains at 0.48 g /g, how much different are the ammonia and oxygen requirements for wild-type E. coli that is unable to synthesize recombinant protein? а. b. с. 24°C 08:39 Berawan 27/04/2022

the image is the steps for help. Not writing assignment!!!! What is the intended outcome of the lysozyme-based ion exchange chromatography described above? Will the protein be effectively separated using the materials listed in the image, or will it fail? Give a brief explanation for your decision.

Give a lab report introduction. introduction should include a general background on bacteriophages, the use of bacteriophages in bacterial genome engineering, and a description of the overall practical aims. Total word count should be no greater than 500 words.

Transcribed Image Text:Complete the following tasks. You discovered that a species of bacteria can break down StyrofoamT (polystyrene) products due to an enzyme it produces, polystyrenase. You wish to study the gene that codes for this enzyme. Task 1: DNA Extraction To begin work on the bacterium, you begin by extracting its genomic DNA (GDNA). What is the purpose of the following procedures? Answer briefly but completely. Using sodium dodecyl sulfate, a detergent Answer: а. b. Adding RNase A and Proteinase K during extraction Answer: c. Adding ethanol before recovering the DNA extract С. Answer: Task 2: Polymerase Chain Reaction After purifying the gDNA extract, you want to isolate and amplify the polystyrenase gene. You perform PCR using the appropriate gene-targeted primers. What is the purpose of the following PCR components? Answer briefly but completely. DNA polymerase isolated from Thermus aquaticus Answer: а. b. Deoxynucleotide triphosphates (DNTPS) Answer: С. Forward and…

available to you). Summarize the results of your research on table 1.4. The first item is given as an example. Table 1.4: Transgenic Organisms and How They Benefit Human Society Field How the Organism Benefits Human Society (Limit your explanation to 2 or 3 sentences.) Because of these transgenic E. coli, human insulin can producing E. coli now be obtained without using human pancreas from cadavers. This also made possible the production of large amounts of human insulin over a short period of time. Allergic reactions from insulin injections are also Transgenic Organism Human insulin- Medicine prevented. Medicine

ersonal/eenongen_my_tnstate_edu/_layouts/15/doc.aspx?sourcedoc={a6b083c9-a226-4c31... ☆ Search (Option + Q) Review View Help Picture Editing A В ... During nucleic acid hybridization, the probe is labelled Question 1 options: for DNA stability to increase probe-test DNA binding to identify the location of probe and the test DNA binding for amplification Question 2 6. 9. 10 IV 13 14 Which of the following best describes the trait in the pedigree? Question 2 options: X-linked dominant X-linked recessive autosomal domiant autosomal recessive ON

Indicate true or false for the following statements   The glycerol used in the DNA loading dye allows DNA to be visualized under UV light. The DNA Ladder used for agarose gel electrophores can be used to estimate fragment size and DNA concentration. During gel electrophoresis a DNA smear may indicate that DNase was still present in the sample.

TOPIC: E. coli T4 (T4 Bacteriophage) Include a maximum of 3 sentences, a brief description of their growth. What are the structures responsible for growth and reproduction? What are their physical and nutritional (or chemical) requirements?

Search (Option + Q) rences Review View Help Editing v A^ Dv A A A Question 1 During nucleic acid hybridization, the probe is labelled for DNA stability to increase probe-test DNA binding to identify the location of probe and the test DNA binding for amplification Question 2 10 IV 12 13 14 Which of the following best describes the trait in the pedigree? Question 2 options: X-linked dominant X-linked recessive autosomal domiant autosomal recessive

UPVOTE WILL BE GIVEN! ANSWER IN 3-5 PARAGRAPHS (TYPEWRITTEN) a. What are the applications of modern biotechnology in the field of engineering? b. What are the implications of these applications? c. How do you think these applications will change the world in the future?

Complete the sentences. Each term may be used more than once. In a circular bacterial chromosome, the structure of DNA is a If DNA is twisted in the Overwinding results in If DNA is twisted in the Underwinding results in One effect of double helix. direction, it becomes overwound. supercoiling. direction, it becomes underwound. supercoiling. double helix. Answer Bank positive negative right-handed left-handed supercoiling in bacterial chromosomes is to promote separation of the two strands of DNA in the

UPVOTE WILL BE GIVEN! ANSWER IN 3-5 PARAGRAPHS (TYPEWRITTEN) a. What are the applications of modern biology in biotechnology? b. What are the implications of these applications? c. How do you think these applications will change the world in the future?

What makes lysozyme and penicillin similar? How are they different? Edit View Insert Format Tools Table

LO 63 Explain how CRISPR-Cas is applied in different technologies Question 14 LO 63- Explain how CRISPR-Cas is applied in different technologies Which of the following functions can be fulfilled by Cas proteins? Create mutations in target DNA Cut RNA Cut sequences at an alternative site Guide CRISPR to the target DNA Form sticky ends to insert genes Cut target DNA RESEARCH ANSWER EXPLANATION

Copy and paste the link below and watch the video on Youtube and Answer the Questionshttps://www.youtube.com/watch?v=g-dNJdOvBM4 Polymerase Chain Reaction  Questions: 1. What are the materials used for the polymerase chain reaction?  2. Draw a schematic diagram of the procedure in PCR.  3. Why is it important to design the primers at the start of the laboratory procedure? 4. What are the components of the PCR buffer and what is its pH range? What is the purpose of the buffer? 5. What is the use for magnesium chloride?  6. How much template DNA is added? What is the concentration of the primers? 7. At what temperatures does denaturation, annealing and extension occur? Why are the processes placed in that temperature? 8. In this particular PCR experiment, how many cycles was used? 9. Can this PCR be used on its own to find out if a person has Covid or not on its own? Why or why not?

question: Can you summarize and explain for me what you want to tell in the article below? Can you explain the figure?  When I read it myself, I do not understand exactly what is meant by the article. It would be nice if you could highlight the important points. You can use them in a figure or diagram to explain. thank you and hava a nice day :) Article:  Nanotechnology Tools to Detect SARS-CoV-2 Standard procedures for detecting the virus from nasopharyngeal and/or oropharyngeal swabs have been reviewed recently and are primarily based on reverse transcription polymerase chain reaction (RT-PCR). Here, we would like to mention some preliminary ideas on nanotechnology-based assays to monitor the presence of SARS-CoV-2. A simplified test and variants thereof to detect viral proteins (e.g., HIV or influenza virus) without the need for expensive equipment is based on the color change of Au NPs bound to antibodies. Similar to the enzyme-linked immunosorbent assay (ELISA) antibodies coupled…

I need help with a biology lab question. What are the four componets required for PCR to work?     thanks

Restriction mapping of the delta chromosome I need help with question two please

Available whis.picture. https://www.phys.ksu.edu/gene/photos/sd.html.g will help vou imagine the results for plate

For letter A, pls ILLUSTRATE (create an illustration or drawing) the DILUTION SERIES of the problem just like the sample on the 2nd image.  Please read the instructions carefully as I have already posted this twice and the experts just copy-pasted the answers from my first post. I don't want to waste another post question for this one. Again, I NEED AN ILLUSTRATION and not just the computation/explanation through words so I can properly visualize the problem. WILL UPVOTE if I get what I need.

Introduction You are a HIV researcher and the following questions depict your struggles while developing, designing and interpreting experiments. All the questions will relate to one another but you do not need to do them in order. Each question will have multiple parts and partial credit will be given whenever possible. Make sure to use your book and notes to help understand the question and develop your answers thoroughly. Question 1 Your interest in HIV started with the protein below the protein below. 1HSG is a dimer and is crystalized with a resolution of 1.9A. The active site is made up each dimer with one monomer in blue and the other in red. What two 2° structures are highlighted (with the black circle) in this figure? The red amino acid sequence is; WKPKMIGGIGGFIKVRQY. What is the most likely sequence of the circled portion? Explain how you choose this sequence You did a BLAST analysis of your POI's and the following were returned as the first hits. 1DIF HIV-1 PROTEASE, 418Z…

ANSWERS 1-3 were answered but I need 4 and 5 answered please. Three parts are solved in case of interlinked question as per our company policy. If you want assistance with other parts, please post separately.  The DNA is made up of deoxyribonucleotide sub units. It is the genetic material. Step 2 The deoxyribonucleotides consist of deoxyribose sugar, phosphate group and nitrogenous bases. Nitrogenous bases are adenine, guanine, thymine and cytosine.  Answer 1) The sequence of template strand in 3' to 5' sequence is: 3' TACCCGCCAGCCTACATC 5' Answer 2)  The mRNA strand is complementary to the template strand. The RNA polymerase synthesises mRNA. The A, U, G and C are present in mRNA with respect to the T, A, C and G in DNA.  The mRNA sequence is: 5' AUGGGCGGUCGGAUGUAG 3' The mRNA sequence in form of codon is: 5' AUG GGC GGU CGG AUG UAG 3' The codon is made up of three nucleotides.  Answer 3) The mRNA codon chart helps to find the sequence of amino acid from a codon. The start…

Instructions: Read 13-2 Manipulating DNA pages 322-323. As you read each section, examine the figures and captions (explanations). Identify any questions you may have. 1) Develop an analogy for the processes researchers use to make changes to DNA. In yo analogy, explain how it is similar to the techniques used in genetic engineering. You can draw a graphic organizer, make a table, or write a few sentences describing your analogy. 2) Devise flowchart that shows the steps to prepare DNA for gel electrophoresis, as well the protocol for setting up and running a gel. You can add diagrams to the flowchart an add detailed notes if you like. English (inited Sate) O Focs ere to search 4 CO RU G\ L B. 2N A\ Alt Ciri

A MLS supervisor is writing an Standard Operating Procedure (SOP) for running a PC test for Hepatitis B DNA. There are several things she must include in the instructions to run the test.1. On average, how many PCR cycles must be run to see if the test is positive or negative for HBV?2. What would a positive test and a negative test look like?3. Her younger sister wants to know what she did at work today. How should she explain what happens during each step of the PCR cycle.

AutoSave O Off # 2 Mol Bio Mutation QUESTIONS - Saved to this Pc - O Search A MS T MCHUNU MT File Home Insert Design Layout References Mailings Review View Help Chemistry A Share P Comments (i SIGN IN TO OFFICE It looks like your stored credentials are out of date. Please sign in as 20*****@ke****** za so we can verify your subscription. Sign In Task B [5 points]: Sanger sequencing Having successfully amplified the NRAS gene from wild-type and cancerous tissue through PCR, you now sent your PCR product for sequencing. However, the only available technology to you is classical Sanger sequencing or the dideoxy chain termination method, explained in Figure 2. Shown below are the sequencing gels obtained for the sections of the wild-type and mutant NRAS genes where the mutation can be found. WILD-TYPE NRAS MUTANT NRAS ddA ddT ddC ddG ddA ddT ddC ddG 1. Determine the 5'-to-3' sequence of the wild-type TEMPLATE strand. Answer: 5' 3' 2. Using the codon table given below, determine the…

Question. Rewrite the following sentences after correction. (Subject: Biotechnology) The variation in the length of tandem repeat of microsatellite DNA has serious translational affects as this is due to its coding region. Correct: If one parent has sickle cell anemia and other has carrier genotype than there is 25 % chance that any offspring is carrier. Correct: Sickled WBC block the flow of blood and Calcium as they stick together and caused by frame shift mutation. Correct: The N1303K mutation in the CFTR gene of CF patients is autosomal dominant disorder due to insertion of asparagine at 1303. Correct: If a person RBCs have B surface antigen and it will clump with antigen B such clumping indicates Blood type B. Correct: Indirect ELISA can detect polygenic gene expression. Correct:

General Instruction: Answer the following questions completely. 1. Construct a Venn Diagram to compare and contrast the structure and function of DNA and RNA. DNA RNA 2. The Continuity of life is based on heritable information in the form of DNA, and structure and function are correlated at all levels of biological organization. Describe how the structure of DNA is correlated with its role as the molecular basis of inheritance. (Answer must be in 3- 5 sentences only).